Reporter expression is observed in nurse cells and in the oocyte, where it becomes

enriched early in oogenesis. During mid-oogenesis, GFPLost is concentrated at the oocyte anterior margin and is also weakly detected at the posterior cortex. In later stage embyros, it is enriched in the cortex both anteriorly and posteriorly. In early embryos, it is concentrated at the posterior pole and is incorporated into pole cells.