FlyBase Allele Report: Dmel\rl[NMG.GMR.Tag:NLS(SV40-largeT),Tag:HSV-1-gD]

General Information

Symbol

Dmel\rl^{NMG.GMR.Tag:NLS(SV40-largeT),Tag:HSV-1-gD}

Species

D. melanogaster

Name

FlyBase ID

FBal0151607

Feature type

allele

Associated gene

Dmel\rl

Associated Insertion(s)

Carried in Construct

P{GMR-rl.NMG}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Kumar et al., 2003)

Progenitor genotype

Carried in construct

P{GMR-rl.NMG}

Cytology

Description

GMR regulatory sequences drive expression of the complete rl coding sequence, followed by amino acids 2-147 of Scer\GAL4 (includes the DNA-binding domain), followed by residues 413-490 of Hsim\VP16 (activation domain), followed by a Tag:HSV-1-gD epitope tag. The fusion protein is tagged at the N-terminal end with a nuclear localisation signal (Tag:NLS(SV40-largeT)).

(Kumar et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Tagged with

Tag:HSV-1-gD

epitope tag

Tag:NLS(SV40-largeT)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Marenda et al., 2006)

Phenotype Manifest In

ommatidium, with rl^Sem

(Kumar et al., 2003)

Detailed Description

Statement

Reference

Induction of rl^{GMR.T:SV40\nls2,T:Scer\GAL4,T:Hsim\VP16,T:Hsim\gD} in larvae for 1 hour, followed by 6 hours recovery, results in a dramatic reduction in the fraction of cells in G1, while greatly raising the fraction in S and G2/M. After 24 hours, the fraction of cells in G2/M rises, at the expense of the pool in G1 and S.

(Marenda et al., 2006)

rl^Sem/+ flies have a rough eye phenotype, which is enhanced if the flies also express rl^{GMR.T:SV40\nls2,T:Scer\GAL4,T:Hsim\VP16,T:Hsim\gD}.

(Kumar et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

rl^{GMR.T:SV40\nls-largeT,T:Scer\GAL4,T:Hsim\VP16,T:Hsim\gD}

rl^{GMR.T:SV40\nls2,T:Scer\GAL4,T:Hsim\VP16,T:Hsim\gD}

rl^{NMG.GMR.Tag:NLS(SV40-largeT),Tag:HSV-1-gD}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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