A ninaE fragment containing 2.5kb of promoter sequence, the transcription start site and 33bp of the 5' untranslated region is fused upstream of Lpol\Ops1 sequences, which consist of 180bp of 5' untranslated sequence and the coding region for amino acids 1-362 (out of 367 in total). The Lpol\Ops1 coding sequence is tagged at the C-terminal end with Tag:Rho1D4. 650bp of the 3' end of the ninaE gene (including the polyadenylation signal) are also present.
Introduction of Lpol\Ops1ninaE.T:Btau\1D4 into a norpA36 ; ninaE17/ninaE17;norpAninaE.PM background restores the response to light (measured using an ERG recording), although the response at 520nm is much great than that at 350nm. Flies expressing Lpol\Ops1ninaE.T:Btau\1D4 in a ninaE17 Gα49B1 background show no response to light (measured using an ERG recording). Flies expressing Lpol\Ops1ninaE.T:Btau\1D4 in a ninaE17 norpA36 background show no response to light (measured using an ERG recording).