FlyBase Allele Report: Dmel\sNPF[RNAi.UAS]

General Information

Symbol

Dmel\sNPF^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0158705

Feature type

allele

Associated gene

Dmel\sNPF

Associated Insertion(s)

Carried in Construct

P{UAS-sNPF.RNAi}

Also Known As

UAS-sNPF-RNAi, UAS-sNPF-Ri

Key Links

Transgenic product class

RNAi_reagent

(Lee et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Lee et al., 2004)

Mutagen

in vitro construct

(Lee et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-sNPF.RNAi}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat of a 0.8kb sNPF cDNA fragment.

(Lee et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

sNPF

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal feeding behavior | adult stage, with Scer\GAL4^MJ94

(Lee et al., 2004)

abnormal feeding behavior | larval stage, with Scer\GAL4^hs.PB

(Lee et al., 2004)

abnormal locomotor behavior | adult stage | female, with Scer\GAL4^c584

(Kahsai et al., 2010)

abnormal locomotor behavior | adult stage | male, with Scer\GAL4^c584

(Kahsai et al., 2010)

abnormal pain response | larval stage, with Scer\GAL4^Ilp7.PY

(Hu et al., 2017)

abnormal pain response | larval stage, with Scer\GAL4^Ilp7.PY, sNPF^c00448

(Hu et al., 2017)

abnormal pain response | larval stage, with Scer\GAL4^Ilp7.PY, sNPF^MI01807

(Hu et al., 2017)

decreased body size, with Scer\GAL4^sNPF.2.4

(Lee et al., 2009)

decreased cell size, with Scer\GAL4^MJ94

(Lee et al., 2008)

long lived, with Scer\GAL4^MJ94

(Lee et al., 2008)

short lived | conditional, with Scer\GAL4^dimm-929

(Kahsai et al., 2010)

short lived | conditional, with Scer\GAL4^Kurs6

(Kahsai et al., 2010)

Phenotype Manifest In

embryonic/larval fat body, with Scer\GAL4^MJ94

(Lee et al., 2008)

Detailed Description

Statement

Reference

Expression of sNPF^dsRNA.UAS under the control of Scer\GAL4^Ilp7.PY, but not Scer\GAL4^ppk.PG, in larvae slightly, but significantly, reduces nociceptive movement responses following stimulation with a von Frey filament, compared to controls; this phenotype is exacerbated in sNPF^MI01807 or sNPF^c00448 heterozygotes.

(Hu et al., 2017)

Females expressing sNPF^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c584 travel longer distances and have a higher mean walking speed than controls in a walking assay. Males expressing sNPF^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c584 travel longer distances in a walking assay, but their mean walking speed is normal.

(Kahsai et al., 2010)

Expressing sNPF^dsRNA.UAS under the control of either Scer\GAL4^dimm-929 or Scer\GAL4^Kurs6 results in a significant reduction in lifespan when flies are deprived of food and water. Expression under the control of Scer\GAL4^Kurs6 also results in a significant reduction in lifespan when flies are deprived of food alone.

(Kahsai et al., 2010)

Expression of sNPF^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sNPF.2.4 decreases body weight and size by 12-18% compared to control siblings.

(Lee et al., 2009)

Expression of sNPF^{dsRNA.Scer\UAS} using Scer\GAL4^MJ94 has little effect on size.

Flies expressing two copies of sNPF^{dsRNA.Scer\UAS} using Scer\GAL4^Ilp2.PR are of similar size to wild type.

Expression of sNPF^{dsRNA.Scer\UAS} using Scer\GAL4^MJ94 decreases the size of fat body cells by 38%.

Expression of sNPF^{dsRNA.Scer\UAS} using Scer\GAL4^MJ94 increases median lifespan by 16-21%.

(Lee et al., 2008)

In adults, expression of sNPF^{dsRNA.Scer\UAS} under the control of Scer\GAL4^MJ94 significantly decreases the fraction of fed flies (assayed by uptake of coloured food fed to the animals for 10 minutes) compared with controls. In third instar feeding larvae, expression of sNPF^{dsRNA.Scer\UAS} under the control of Scer\GAL4^hs.PB significantly decreases the fraction of fed larvae (assayed by uptake of coloured food fed to the animals for 10 minutes) compared with controls. In wandering larvae, expression of sNPF^{dsRNA.Scer\UAS} under the control of Scer\GAL4^unspecified has no effect on the fraction of fed larvae (assayed by uptake of coloured food) compared with controls. Adult flies expressing sNPF^{dsRNA.Scer\UAS} under the control of Scer\GAL4^MJ94 do not show a difference in size and weight compared to control flies.

(Lee et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sNPF^RNAi.UAS

sNPF^{dsRNA.Scer\UAS}

sNPF^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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