investment cone (with mlt1)
investment cone (with mlt2)
Individualisation complexes assembled around spermatid nuclear bundles are intact in homozygous, mltEY02157/mlt1, mltEY02157/mlt2, mltEY02157/mlt4210, mltG18151/mltEY02157 and mltEY02157/Df(2R)BSC281 testes, but progressed individualisation complexes (those no longer co-localising with the nuclear bundle) are often disrupted, having one or more investment cones out of register. Sleeves of F-actin associated with highly condensed spermatid bundles at the most basal end of the testis tubule are sometimes seen.
Individualisation complexes assembled around spermatid nuclear bundles are intact in mltEY02157/Df(2R)stan2 testes, but progressed individualisation complexes (those no longer co-localising with the nuclear bundle) are often disrupted, having one or more investment cones out of register.
Tubulin staining in mltEY02157/Df(2R)BSC281 and mltEY02157/Df(2R)BSC350 mutant spermatogenic cysts is normal prior to individualisation complex movement, but in contrast to wild type, tubulin staining does not disappear from mutant cysts upon progression of individualisation complex.
Although the P{EPgy2}mltEY02157 insertion maps within both the CG12214 annotation and an intron of KCNQ (nested genes, CG12214 is entirely within a large intron of KCNQ), complementation analysis indicates that the "mlt" mutant phenotype caused by this insertion (spermatid individualisation defects) is due to an effect on CG12214 and not on KCNQ.