FlyBase Allele Report: Dmel\udt[f03018]

General Information

Symbol

Dmel\udt^f03018

Species

D. melanogaster

Name

FlyBase ID

FBal0162714

Feature type

allele

Associated gene

Dmel\udt

Associated Insertion(s)

PBac{WH}udt^f03018

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{WH}udt^f03018

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:23,762,495..23,762,495 [-]

(Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage

(Petri et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

udt^f03018 homozygous and udt^f03018/udt^Δ transheterozygous stage 16-17 embryos show leaky blood-brain barrier and trachea as, unlike in controls, dye injected into the body cavity diffuses into the ventral nerve cord and into the trachea. Although the ventral nerve cord show an apparently normal morphology, the trachea appears convoluted, as compared to controls.

The trachea of udt^f03018 homozygous stage 16 embryos does not seem to form septate junctions, as the associated proteins NrxIV, Cora and Dlg1 are found all along the basolateral membrane instead of restricted to the apical cell-cell contact domains. Likewise, in stage 17 embryo epidermis and hindgut cells NrxIV is also mislocalized and septate junction strands are not visible at the ultrastructural level. These defects do not seem to be associated with overall cell polarity defects, as those protein are never found in the apical domain and the distribution of the adherens junction protein E-Cad is unchanged.

udt^f03018 homozygous clones in the first-instar wing imaginal disc are numerous and well integrated into the imaginal tissue; these seem to have some septate junction.

(Petri et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

udt^f03018 is rescued by udt^UAS.cPa/Scer\GAL4^da.G32

(Petri et al., 2019)

udt^f03018 is rescued by Scer\GAL4^en-e16E/udt^UAS.cPa

(Petri et al., 2019)

udt^f03018 is rescued by Scer\GAL4^da.G32/udt^{ΔGPI.UAS.Tag:HA}

(Petri et al., 2019)

udt^f03018 is rescued by Scer\GAL4^en-e16E/udt^{ΔGPI.UAS.Tag:HA}

(Petri et al., 2019)

udt^f03018 is rescued by udt^{UAS.Tag:MYC,Tag:SS(rCd2)}/Scer\GAL4^da.G32

(Petri et al., 2019)

udt^f03018 is rescued by udt^{ΔGPI.UAS.Tag:MYC,Tag:SS(rCd2)}/Scer\GAL4^da.G32

(Petri et al., 2019)

udt^f03018 is rescued by Scer\GAL4^da.G32/udt^UAS.Tag:HA

(Petri et al., 2019)

udt^f03018 is rescued by Scer\GAL4^da.G32/udt^3xA.UAS.HA

(Petri et al., 2019)

udt^f03018 is rescued by udt^{TM.UAS.Tag:HA,Tag:M(mCd8a)}/Scer\GAL4^da.G32

(Petri et al., 2019)

udt^f03018 is rescued by udt^{TM.UAS.Tag:HA,Tag:M(mCd8a),EGFP}/Scer\GAL4^da.G32

(Petri et al., 2019)

udt^f03018 is rescued by Scer\GAL4^da.G32/udt^{TM.UAS.Tag:MYC,Tag:SS(rCd2),Tag:M(mCd8a)}

(Petri et al., 2019)

Partially rescued by

udt^f03018 is partially rescued by udt^UAS.cPa/Scer\GAL4^repo

(Petri et al., 2019)

udt^f03018 is partially rescued by Scer\GAL4^repo/udt^{ΔGPI.UAS.Tag:HA}

(Petri et al., 2019)

udt^f03018 is partially rescued by udt^{UAS.Tag:MYC,Tag:SS(rCd2)}/Scer\GAL4^repo

(Petri et al., 2019)

udt^f03018 is partially rescued by Scer\GAL4^en-e16E/udt^{UAS.Tag:MYC,Tag:SS(rCd2)}

(Petri et al., 2019)

udt^f03018 is partially rescued by udt^{ΔGPI.UAS.Tag:MYC,Tag:SS(rCd2)}/Scer\GAL4^repo

(Petri et al., 2019)

udt^f03018 is partially rescued by Scer\GAL4^en-e16E/udt^{ΔGPI.UAS.Tag:MYC,Tag:SS(rCd2)}

(Petri et al., 2019)

udt^f03018 is partially rescued by Scer\GAL4^repo/udt^UAS.Tag:HA

(Petri et al., 2019)

udt^f03018 is partially rescued by Scer\GAL4^en-e16E/udt^UAS.Tag:HA

(Petri et al., 2019)

udt^f03018 is partially rescued by Scer\GAL4^repo/udt^3xA.UAS.HA

(Petri et al., 2019)

udt^f03018 is partially rescued by Scer\GAL4^en-e16E/udt^3xA.UAS.HA

(Petri et al., 2019)

udt^f03018 is partially rescued by udt^{TM.UAS.Tag:HA,Tag:M(mCd8a)}/Scer\GAL4^repo

(Petri et al., 2019)

udt^f03018 is partially rescued by udt^{TM.UAS.Tag:HA,Tag:M(mCd8a),EGFP}/Scer\GAL4^repo

(Petri et al., 2019)

udt^f03018 is partially rescued by Scer\GAL4^en-e16E/udt^{TM.UAS.Tag:HA,Tag:M(mCd8a),EGFP}

(Petri et al., 2019)

udt^f03018 is partially rescued by Scer\GAL4^repo/udt^{TM.UAS.Tag:MYC,Tag:SS(rCd2),Tag:M(mCd8a)}

(Petri et al., 2019)

Not rescued by

udt^f03018 is not rescued by Scer\GAL4^en-e16E/udt^{TM.UAS.Tag:HA,Tag:M(mCd8a)}

(Petri et al., 2019)

udt^f03018 is not rescued by Scer\GAL4^en-e16E/udt^{TM.UAS.Tag:MYC,Tag:SS(rCd2),Tag:M(mCd8a)}

(Petri et al., 2019)

Comments

The expression of either udt^UAS.cPa or udt^{ΔGPI.UAS.Tag:HA} under the control of either Scer\GAL4^da.G32 or Scer\GAL4^en-e16E rescues both the viability and blood-brain and trachea barrier defects observed in udt^f03018 homozygotes; Scer\GAL4^en-e16E>udt^UAS.cPa also rescues the mislocalization of the septate junction protein NrxIV in the stage 17 embryo hindgut. Their expression under the control of Scer\GAL4^repo only rescues the blood-brain barrier defect, as it does not rescue viability and the trachea is still leaky. Scer\GAL4^en-e16E>udt^{ΔGPI.UAS.Tag:HA} also rescues the mislocalization of the septate junction protein NrxIV in the stage 17 embryo epidermis and hindgut.

The expression of either udt^{UAS.Tag:MYC,Tag:SS(rCd2)} or udt^{ΔGPI.UAS.Tag:MYC,Tag:SS(rCd2)} under the control of Scer\GAL4^da.G32 rescues both the viability and blood-brain and trachea barrier defects observed in udt^f03018 homozygotes. Their expression under the control of either Scer\GAL4^repo or Scer\GAL4^en-e16E only rescues the blood-brain barrier defect, as it does not rescue viability and the trachea is still leaky.

The expression of either udt^UAS.Tag:HA or udt^3xA.UAS.HA under the control of Scer\GAL4^da.G32 rescues both the viability and blood-brain and trachea barrier defects observed in udt^f03018 homozygotes. Their expression under the control of Scer\GAL4^repo only rescues the blood-brain barrier defect, as it does not rescue viability and the trachea is still leaky. Their expression under the control of Scer\GAL4^en-e16E only partially rescues the blood-brain barrier defect, as it does not rescue viability and the trachea is still leaky. Scer\GAL4^en-e16E>udt^UAS.Tag:HA and Scer\GAL4^en-e16E>udt^3xA.UAS.HA also rescue the mislocalization of the septate junction protein NrxIV in the stage 17 embryo epidermis and hindgut.

The expression of udt^{TM.UAS.Tag:HA,Tag:M(mCd8a)}, udt^{TM.UAS.Tag:HA,Tag:M(mCd8a),EGFP} or udt^{TM.UAS.Tag:MYC,Tag:SS(rCd2),Tag:M(mCd8a)} under the control of Scer\GAL4^da.G32 rescues both the viability and blood-brain and trachea barrier defects observed in udt^f03018 homozygotes. Their expression under the control of Scer\GAL4^repo only rescues the blood-brain barrier defect, as it does not rescue viability and the trachea is still leaky. Their expression under the control of Scer\GAL4^en-e16E fails to rescue viability as well as the blood-brain barrier and trachea barrier defects. Scer\GAL4^en-e16E>udt^{TM.UAS.Tag:HA,Tag:M(mCd8a),EGFP}, however, rescues the mislocalization of the septate junction protein NrxIV in the stage 17 embryo epidermis and hindgut, but not in the trachea.

(Petri et al., 2019)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

18607

w¹¹¹⁸; PBac{WH}udt^f03018/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG10217^P[f03018]

(Petri et al., 2019)

CG10217^f03018

udt^f03018

undicht^f03018

(Petri et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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