FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\udtTM.UAS.Tag:HA,Tag:M(mCd8a),EGFP
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General Information
Symbol
Dmel\udtTM.UAS.Tag:HA,Tag:M(mCd8a),EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0346107
Feature type
allele
Associated gene
Dmel\udt
Associated Insertion(s)
Carried in Construct
M{UAS-udt.HA.TMeGFP}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Petri et al., 2019)
Progenitor genotype
Carried in construct
M{UAS-udt.HA.TMeGFP}
Cytology
Description

UASt regulates expression of the cDNA LD02314 with a C-terminal Tag:HA-tag (including two additional amino acids) just N-terminal to the GPI attachment site, and in which the GPI attachment site is replaced with the transmembrane domain from mCD8 (Tag:M(mCd8a)) and an EGFP tag.

(Petri et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
udt
Tagged with
EGFP
Tag:HA
Tag:M(mCd8a)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      udtTM.UAS.Tag:HA,Tag:M(mCd8a),EGFP/Scer\GAL4da.G32 rescues udtf03018

      (Petri et al., 2019)
      Partially rescues

      udtTM.UAS.Tag:HA,Tag:M(mCd8a),EGFP/Scer\GAL4repo partially rescues udtf03018

      (Petri et al., 2019)

      Scer\GAL4en-e16E/udtTM.UAS.Tag:HA,Tag:M(mCd8a),EGFP partially rescues udtf03018

      (Petri et al., 2019)
      Comments

      The expression of udtTM.UAS.Tag:HA,Tag:M(mCd8a),EGFP under the control of Scer\GAL4da.G32 rescues both the viability and blood-brain and trachea barrier defects observed in udtf03018 homozygotes. Expression under the control of Scer\GAL4repo only rescues the blood-brain barrier defect, as it does not rescue viability and the trachea is still leaky. Expression under the control of Scer\GAL4en-e16E fails to rescue viability as well as the blood-brain barrier and trachea barrier defects; this expression, however, rescues the mislocalization of the septate junction protein NrxIV in the stage 17 embryo epidermis and hindgut, but not in the trachea.

      (Petri et al., 2019)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      udtTM.UAS.Tag:HA,Tag:M(mCd8a),EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)