The FRT cassette has been excised, removing GFP and y markers, leaving a UAS driven mutated smo sequence (carries amino acid replacements S667A, S687A and S740A) that is tagged with a single copy of Tag:HA at the C-terminus.
Expression of smoPKA.Scer\UAS.T:Ivir\HA1, under the regulation of Scer\GAL4Bx-MS1096 produces wings with reduced territory between veins 3 and 4, as well as partial fusions of those veins.