The FRT cassette has been excised, removing GFP and y markers, leaving a UAS driven mutated smo sequence (carries amino acid replacement S683A) that is tagged with a single copy of Tag:HA at the C-terminus.
Expression of smoGSK3.Scer\FRT.Avic\GFP.y.Scer\UAS.T:Ivir\HA1 under the regulation of Scer\GAL4Bx-MS1096 does not disturb the adult wing pattern.