FlyBase Allele Report: Dmel\Rab3-GAP[c04953]

General Information

Symbol

Dmel\Rab3-GAP^c04953

Species

D. melanogaster

Name

FlyBase ID

FBal0178255

Feature type

allele

Associated gene

Dmel\Rab3-GAP

Associated Insertion(s)

PBac{PB}Rab3-GAP^c04953

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

PBac{PB}Rab3-GAP^c04953

Cytology

Description

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:12,063,116..12,063,116 [-]

(Müller et al., 2011, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | chemical sensitive

(Müller et al., 2011)

abnormal neurophysiology | chemical sensitive (with Df(2L)ED775)

(Müller et al., 2011)

abnormal neurophysiology | third instar larval stage

(Younger et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Active zone (brp area) size is similar to wild type at the neuromuscular junction (NMJ) of Rab3-GAP^c04953/Df(2L)ED775 third instar larvae.

(Chen et al., 2015)

Mutant third instar larvae show no significant increase in quantal content at the neuromuscular junction in the presence of the glutamate receptor antagonist philanthotoxin-433 (10μM), indicating a defect in synaptic homeostasis.

(Younger et al., 2013)

The average mEPSP amplitude, EPSP amplitude, and quantal content in rab3-GAP^c04953 mutant synapses do not differ from wild-type.

Bath application of 10 υM philanthotoxin-433 (PhTX) induced a significant decrease in mEPSP amplitude in both wild-type and rab3-GAP^c04953 mutant synapses. However, unlike in wild-type, there is no homeostatic increase in quantal content following application of PhTX to the neuromuscular junction in rab3-GAP^c04953 mutants as in wildtype. A similar block in synaptic homeostasis is observed when rab3-GAP^c04953 is placed in trans with Df(2L)ED775, which uncovers the rab3-GAP locus. There is no apparent defect in baseline synaptic transmission in rab3-GAP^c04953/Df(2L)ED775 mutant animals. However, following PhTX application, the average mEPSP amplitude and average EPSP amplitude are significantly decreased in rab3-GAP^c04953/Df(2L)ED775 mutants and quantal content remains unchanged.

The input resistance of rab3-GAP^c04953 mutant muscles is significantly smaller than that observed in wild-type muscles.

There is no apparent difference in active zone density or bouton number between rab3-GAP^c04953 mutant and wild-type neuromuscular junctions.

A 20Hz stimulus train induces synaptic depression in both wild-type and rab3-GAP^c04953 mutants. The average steady-state EPSP amplitude is slightly, but statistically significantly larger at rab3-GAP^c04953 synapses as compared to wild-type. There is no significant difference in the EPSP amplitude elicited by the first stimulus of a train between rab3-GAP^c04953 mutants and wild-type. Whereas quantal content at an extracellular "["CA[2+]"]" of 0.5mm is no different in rab3-GAP^c04953 mutants compared to wild-type, quantal content is significantly decreased at 0.2mM "["ca[2+]"]" in rab3-GAP^c04953 mutants. There is a decrease in presynaptic release probability at rab3-GAP^c04953 mutant synapses that is revealed at low extracellular calcium concentrations.

The difference in EPSP amplitude between rab3-GAP^c04953 mutants and wild-type becomes less pronounced during the course of a 20Hz stimulus train at low extracellular "["Ca[2+]"]". On average, the EPSP amplitude of rab3-GAP^c04953 mutants, which is significantly smaller in response to the first few stimuli, reaches the level of wild-type EPSPs after approximately the first 20-25 stimuli of a 20Hz train. However, rab3-GAP^c04953 mutant EPSPs remain significantly smaller than the amplitude of wild-type EPSPs if the stimulation frequency is reduced to 10Hz.

(Müller et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rab3-GAP^c04953 has abnormal neurophysiology phenotype, enhanceable by GluRIIA^SP16

(Müller et al., 2011)

Rab3-GAP^c04953 has abnormal neurophysiology | chemical sensitive phenotype, enhanceable by Rab3^rup

(Müller et al., 2011)

Rab3-GAP^c04953 has abnormal neurophysiology phenotype, enhanceable by Rab3^rup

(Müller et al., 2011)

NOT Suppressor of

Statement

Reference

Rab3-GAP^c04953 is a non-suppressor of abnormal neurophysiology phenotype of Rab3^rup

(Müller et al., 2011)

Other

Statement

Reference

Rab3-GAP^c04953, Rab3^rup has abnormal neurophysiology phenotype

(Müller et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Philanthotoxin-433 (PhTX) causes a significant decrease in mEPSP amplitude in rab3-GAP^c04953 Rab3^rup double mutants. A significant, nearly 2-fold increase in quantal content is observed following PhTX treatment in the double mutant compared to controls, indicating that rab3-GAP^c04953 Rab3^rup double mutants undergo synaptic homeostasis.

rab3-GAP^c04953 Rab3^rup double mutants exhibit major defects in baseline transmission.

rab3-GAP^c04953 Rab3^rup double mutants exhibit synapse morphology defects that are identical to those observed in Rab3^rup alone.

GluRIIA^SP16, rab3-GAP^c04953 double mutants exhibit significantly reduces mEPSP amplitudes compared to rab3-GAP^c04953 mutant synapses alone. As a consequence, the mean EPSP amplitude of GluRIIA^SP16, rab3-GAP^c04953 double mutant neuromuscular junctions is significantly smaller than the EPSP amplitude records in rab3-GAP^c04953 mutants alone.

(Müller et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rab3-GAP^c04953 is rescued by Scer\GAL4^elav-C155/Rab3-GAP^UAS.ECFP

(Müller et al., 2011)

Comments

A homeostatic increase in presynaptic quantal content is observed following application of PhTX to rab3-GAP^c04953 mutants expressing rab3-GAP^{Scer\UAS.T:Avic\GFP-YFP.Venus} pre-synaptically under the control of Scer\GAL4^elav-C155. The observed increase in quantal content offsets the PhTX-dependent decrease in mEPSP amplitude and restores EPSP amplitudes to baseline values. However, the decrease in input resistance found in rab3-GAP^c04953 mutant muscles remains.

(Müller et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

85971

w¹¹¹⁸; PBac{PB}Rab3-GAP^c04953

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rab3-GAP^c04953

rab3-GAP^c04953

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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