The org-1 N, bi T and org-1 C domains have been fused in order separated by two or three junctional amino acids (A,G or T). Tag:HA tags are at the C and N termini. Expression of this construct is driven by UASt sequences.
eye, with Scer\GAL4dpp.blk1
ommatidium, with Scer\GAL4GMR.PF
When bi::org-1org-1N+biT+org-1C.Scer\UAS.T:Ivir\HA1 is driven by Scer\GAL4dpp.blk1 large eyes are seen that are expanded caudally. When bi::org-1org-1N+biT+org-1C.Scer\UAS.T:Ivir\HA1 is driven by Scer\GAL4GMR.PF ommatidial development is impaired.