Homozygous Dic61Bc05439 mutants show developmental delay and eclose 24 to 36 hours later than wild type. This phenotype is seen at both 22[o]C and 25[o]C. No defects are seen in the tergites or sternites.
Dic61Bc05439 mutants exhibit pupal lethality when raised at 29[o]C. Approximately 20% of homozygotes and 55% of heterozygotes eclose as flies.
Dic61Bc05439 mutant testes are packed with abnormally bent sperm bundles, making the testes bulge slightly in the middle region and displacing all other cell types from their orderly arrangement. The individualisation complexes are well-organised, but actin cones appear disrupted soon after commencing the journey towards the caudal end of sperm tails and degrade prematurely in the middle region of the testis, before the sperm are individualised.
Dic61Bc05439 fails to complement the male sterile phenotype seen in Dic61Bms21.
Dic61Bc05439 fails to complement the male sterile phenotype seen in either Df(3L)ED201 or Df(3L)Exel6083.
Precise excision of the PBac{PB} transposon in PBac{PB}Dic61Bc05439 rescues the male sterility.