UASt sequences drive expression of a Hsap\RUNX1 fragment (nucleotides 1-531), tagged at the 5' end with a nuclear localization signal. The expressed Hsap\RUNX1 product includes the RD domain.
Expression of Hsap\RUNX1ΔETO.Scer\UAS.T:Zzzz\nls1 under the control of Scer\GAL4lz-gal4 does not impair crystal cell formation.
Scer\GAL4lz-gal4>Hsap\RUNX1ΔETO.Scer\UAS.T:Zzzz\nls1 animals hatch and their eyes appear wild type.