FlyBase Allele Report: Dmel\EcR[RNAi.A.UAS]

General Information

Symbol

Dmel\EcR^RNAi.A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0190780

Feature type

allele

Associated gene

Dmel\EcR

Associated Insertion(s)

Carried in Construct

P{UAS-EcR.A.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Roignant et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Roignant et al., 2003)

Mutagen

in vitro construct

(Roignant et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-EcR.A.RNAi}

Cytology

Description

UASt sequences drive expression of a 646bp fragment of EcR-A (nts 424-1070 of GenBank Accession S63761) organised as an inverted repeat.

(Roignant et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

EcR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult doublesex pC1 (female) neuron | adult stage, with Hsap\RELA^AD.VT002064, Scer\GAL4^{dsx-GAL4(DBD)::Zip-}

(Li et al., 2024)

adult doublesex pC1 (female) neuron | P-stage, with Hsap\RELA^AD.VT002064, Scer\GAL4^{dsx-GAL4(DBD)::Zip-}

(Li et al., 2024)

wing, with Scer\GAL4^Bx-MS1096

(Gradilla et al., 2011)

Detailed Description

Statement

Reference

Expression of EcR^{dsRNA.A.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 results in defects in wing morphology.

(Gradilla et al., 2011)

Expression of EcR^{dsRNA.A.Scer\UAS} under the control of Scer\GAL4^Hml.Δ has no effect on hemocyte proliferation in third instar larvae.

(Sinenko et al., 2010)

Induction of EcR^{dsRNA.A.Scer\UAS}, under the control of either Scer\GAL4^how-24B or Scer\GAL4^NP5169 (and Scer\GAL80^ts.αTub84B, placed at the restrictive temperature of 29^oC at the onset of metamorphosis) dramatically inhibits heart remodelling. The cardiac tube retains its larval morphology, with the characteristic division into aorta in segments A1 to A4 and heart in segments A5 to A7, with A5 myofibrils retaining their larval circular orientation. Furthermore, segment A5 innervations are prevented following EcR^{dsRNA.A.Scer\UAS} induction, while segments A6 and A7 are not eliminated and ventral imaginal muscles do not form.

(Monier et al., 2005)

When EcR^{dsRNA.A.Scer\UAS} is driven by Scer\GAL4^da.G32, animals die as pharate adults.

(Roignant et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

EcR^RNAi.A.UAS, Scer\GAL4^Hml.Δ is an enhancer of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

EcR^RNAi.A.UAS, Scer\GAL4^Hml.Δ is an enhancer of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of EcR^{dsRNA.A.Scer\UAS} slightly enhances the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9328

w¹¹¹⁸; P{UAS-EcR.A.RNAi}91/TM3, P{ActGFP}JMR2, Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

EcR.A^IR

(Zhang et al., 2015)

EcR^RNAi.A.UAS

EcR^{dsRNA.A.Scer\UAS}

EcR^dsRNA.A.UAS

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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