A mib1 cDNA fragment corresponding to nucleotides 3626-4027 is inserted in opposite directions into a pUAST-RNAi intron vector.
Wings derived from a mib1dsRNA.Scer\UAS/Scer\GAL4nub-AC-62 wing imaginal disc, in which mib1dsRNA.Scer\UAS is expressed uniformly throughout the prospective wing, exhibit severe notching and vein thickening.