FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Avic\GFPSCAT3.UAS
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General Information
Symbol
Avic\GFPSCAT3.UAS
Species
A. victoria
Name
FlyBase ID
FBal0191435
Feature type
allele
Associated gene
Avic\GFP
Associated Insertion(s)
Carried in Construct
P{UAS-SCAT3}
Also Known As
UAS-SCAT3
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Kanuka et al., 2005, Kanuka et al., 2005)
Progenitor genotype
Carried in construct
P{UAS-SCAT3}
Cytology
Description

UASt sequences drive expression of "SCAT3", a genetically encoded reporter for caspase activation. SCAT3 comprises the ECFP and Venus proteins linked by an 18 amino acid peptide that includes DEVD, the caspase-3 cleavage sequence. Caspase activation can be monitored by changes in the fluorescence energy transfer (FRET) from ECFP to Venus; cleavage of the linker peptide by activated caspase abolishes the FRET (details from PMID:12527749).

(Kanuka et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Avic\GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      This construct is used to detect caspase activity as cleavage of the amino acid linker separates the two fluorescent markers and leads to a decrease in FRET.

      (Kanuka et al., 2005)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Avic\GFPSCAT3.Scer\UAS
      Avic\GFPSCAT3.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (8)