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General Information
Symbol
Avic\GFPSCAT3.UAS
Species
A. victoria
Name
FlyBase ID
FBal0191435
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-SCAT3
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt sequences drive expression of "SCAT3", a genetically encoded reporter for caspase activation. SCAT3 comprises the ECFP and Venus proteins linked by an 18 amino acid peptide that includes DEVD, the caspase-3 cleavage sequence. Caspase activation can be monitored by changes in the fluorescence energy transfer (FRET) from ECFP to Venus; cleavage of the linker peptide by activated caspase abolishes the FRET (details from PMID:12527749).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

This construct is used to detect caspase activity as cleavage of the amino acid linker separates the two fluorescent markers and leads to a decrease in FRET.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Avic\GFPSCAT3.Scer\UAS
Avic\GFPSCAT3.UAS
Name Synonyms
Secondary FlyBase IDs
    References (8)