FlyBase Allele Report: Dmel\Diap2[RNAi.UAS.cKa]

General Information

Symbol

Dmel\Diap2^RNAi.UAS.cKa

Species

D. melanogaster

Name

FlyBase ID

FBal0191697

Feature type

allele

Associated gene

Dmel\Diap2

Associated Insertion(s)

Carried in Construct

P{UAS-Diap2.RNAi.K}

Key Links

Transgenic product class

RNAi_reagent

(Kleino et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Kleino et al., 2005)

Mutagen

in vitro construct

(Kleino et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Diap2.RNAi.K}

Cytology

Description

UASt sequences drive expression of an inverted repeat, in a head-to-head orientation, corresponding to the first 500bp of the Diap2 coding sequence. A 282bp-long genome fragment of the Ret gene, which contains introns 5 and 6 of this gene, lies between the two Diap2 fragments to enhance the RNAi effect.

(Kleino et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Diap2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^c564

(Kleino et al., 2005)

lethal - all die before end of pupal stage, with Scer\GAL4^da.G32

(Kleino et al., 2005)

some die during pupal stage, with Scer\GAL4^da.G32

(Kleino et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies that express Iap2^{dsRNA.Scer\UAS} in the fat body, under the control of Scer\GAL4^c564, show no detectable defects. However, the immune response to infection with the Gram-negative bacteria P.carotovorum, as determined by induction of Dpt, is strongly reduced.

(Kleino et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Transcription from the P{UAS-Iap2.dsRNA} transgenic construct produces Iap2 dsRNA, resulting in dsRNA interference (RNAi) of the Iap2 gene.

(Kleino et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Diap2^RNAi.UAS.cKa

Diap2^{dsRNA.Scer\UAS}

Diap2^dsRNA.UAS

Iap2^{dsRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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