FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Zzzz\ZFNhs.bwB
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General Information
Symbol
Zzzz\ZFNhs.bwB
Species
a. artificial
Name
FlyBase ID
FBal0193179
Feature type
allele
Associated gene
Zzzz\ZFN
Associated Insertion(s)
Carried in Construct
P{hs-bwB}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Beumer et al., 2006)
Progenitor genotype
Carried in construct
P{hs-bwB}
Cytology
Description

An Hsp70 promoter drives expression of a hybrid zinc finger nuclease, which consists of a DNA-binding domain composed of zinc fingers linked to a nonspecific DNA-cleavage domain derived from the Poke\FokI restriction endonuclease. The zinc finger domains have been designed to recognise the target sequence 5'-GAGGTGGGC-3' which is within bw exon 7.

(Beumer et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
Zzzz\ZFN
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies carrying Zzzz\ZFNhs.bwB are unable to withstand heat-shock temperatures of greater than 35oC.

      (Beumer et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Zzzz\ZFNhs.bwA and Zzzz\ZFNhs.bwB are used in combination to induce double strand breaks within the endogenous bw gene sequence.

      (Beumer et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Zzzz\ZFNhs.bwB
      bwB
      (Beumer et al., 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)