FlyBase Allele Report: Dmel\Ptpmeg[1]

General Information

Symbol

Dmel\Ptpmeg¹

Species

D. melanogaster

Name

FlyBase ID

FBal0193794

Feature type

allele

Associated gene

Dmel\Ptpmeg

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Whited et al., 2007)

I-SceI

(Whited et al., 2007)

FLPase

(Whited et al., 2007)

gene targeting by homologous recombination

(Whited et al., 2007)

Progenitor genotype

Cytology

Description

A 4bp insertion, GTAC, has been introduced into the Ptpmeg gene. This produces a frameshift, which leads to truncation within the PDZ domain.

(Whited et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior

(Whited et al., 2007)

abnormal cell migration (with Df(3L)ED201)

(Li et al., 2015)

abnormal cell migration | recessive

(Li et al., 2015)

abnormal neuroanatomy (with Df(3L)ED201)

(Whited et al., 2007)

abnormal neuroanatomy | recessive

(Whited et al., 2007)

fertile

(Whited et al., 2007)

viable

(Whited et al., 2007)

Phenotype Manifest In

adult mushroom body alpha-lobe

(Whited et al., 2007)

adult mushroom body alpha-lobe (with Df(3L)ED201)

(Whited et al., 2007)

adult mushroom body beta-lobe

(Whited et al., 2007)

axon & ellipsoid body

(Whited et al., 2007)

axon & ellipsoid body (with Df(3L)ED201)

(Whited et al., 2007)

axon & mushroom body | somatic clone | cell non-autonomous

(Whited et al., 2007)

border follicle cell

(Li et al., 2015)

border follicle cell (with Df(3L)ED201)

(Li et al., 2015)

medial lobe of adult mushroom body

(Whited et al., 2007)

vertical lobe of adult mushroom body

(Whited et al., 2007)

Detailed Description

Statement

Reference

Homozygous Ptpmeg¹ causes accelerated migration of border cells during oogenesis.

(Li et al., 2015)

Ptpmeg¹ adults often become trapped alive in their food.

Ptpmeg¹ mutants show mushroom lobe defects. The alpha lobes are reduced, appearing thin, short or with a thin tip. In a small proportion of mutants, the alpha lobes are missing altogether. Tthe beta lobes often aberrantly touch the midline or are completely fused with the contralateral beta lobe. These defects are recessive and cell body and dendritic regions of mushroom body neurons appear normal.

An increase in mushroom body defects occurs as development progresses in Ptpmeg¹ mutants; from 0% to ~55% in dorsal lobes and from ~10% to ~80% in medial lobes between 48 hours post-pupal formation (PPF) and the adult stage. A similar increase in defects can be observed in Ptpmeg¹/Df(3L)ED201 mutants. At 24 hours PPF no significant level of defects can be observed but ~15-20% of dorsal lobes exhibit defects at 36 hours and 48 hours PPF and nearly 50% of mutants show defects by the first day of adulthood. In addition to the reduction in the distal region of dorsal lobes, dots of axonal material can frequently be observed near these regions, suggesting that axons retract in the dorsal lobes of Ptpmeg¹/Df(3L)ED201 mutants.

Ptpmeg¹ mutant clones containing either 1-10 or 10-50 mutant α/β cells do not show mushroom body axon defects. However, clones containing substantial amounts of Ptpmeg¹ brain tissue outside of the mushroom bodies do show mushroom body axon defects.

In Ptpmeg¹ mutants, ellipsoid body axons reach the midline but their extension toward ventral regions halts prematurely, leaving an omega-shaped ellipsoid body that is open along its ventral aspect. These ellipsoid body axon defects persist into adulthood and can also be observed in Ptpmeg¹/Df(3L)ED201 mutants.

(Whited et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Ptpmeg¹ has mushroom body phenotype, suppressible by scc^P

(Whited et al., 2007)

Ptpmeg¹ has ellipsoid body phenotype, suppressible by scc^P

(Whited et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

scc^P is able to suppress the mushroom body and ellipsoid body phenotypes of Ptpmeg¹ mutants.

(Whited et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Ptpmeg¹ is partially rescued by Ptpmeg^UAS.cWa/Scer\GAL4^elav.PLu

(Whited et al., 2007)

Ptpmeg¹ is partially rescued by Ptpmeg^ΔFERM.UAS/Scer\GAL4^elav.PLu

(Whited et al., 2007)

Ptpmeg¹ is partially rescued by Ptpmeg^{G494A.F494A.UAS}/Scer\GAL4^elav.PLu

(Whited et al., 2007)

Ptpmeg¹/Df(3L)ED201 is partially rescued by Ptpmeg^UAS.cWa/Scer\GAL4^elav.PLu

(Whited et al., 2007)

Not rescued by

Ptpmeg¹ is not rescued by Ptpmeg^UAS.cWa/Scer\GAL4^EB1

(Whited et al., 2007)

Ptpmeg¹ is not rescued by Scer\GAL4^elav.PLu/Ptpmeg^C877S.UAS

(Whited et al., 2007)

Ptpmeg¹ is not rescued by Scer\GAL4^elav.PLu/Ptpmeg^R883M.UAS

(Whited et al., 2007)

Ptpmeg¹ is not rescued by Ptpmeg^{Y650F.D787A.UAS}/Scer\GAL4^elav.PLu

(Whited et al., 2007)

Comments

Expression of Ptpmeg^Scer\UAS.cWa under the control of Scer\GAL4^elav.PLu fully rescues the alpha lobe defects and partially rescues the beta lobe defects of Ptpmeg¹ homozygotes. Scer\GAL4^elav.PLu>Ptpmeg^Scer\UAS.cWa expression partially rescues the alpha lobe defects of Ptpmeg¹/Df(3L)ED201 mutants.

Expression of Ptpmeg^Scer\UAS.cWa under the control of Scer\GAL4^elav.PLu rescues the ellipsoid body defects of Ptpmeg¹ mutants but expression of this transgene within the ellipsoid body itself, under the control of Scer\GAL4^EB1, fails to rescue these defects.

Expression of Ptpmeg^{ΔFERM.Scer\UAS} under the control of Scer\GAL4^elav.PLu results in a strong rescue of the ellipsoid body defects of Ptpmeg¹ mutants, a rescue of the beta lobe overextension phenotype but no rescue of the alpha lobe reduction.

Expression of Ptpmeg^{G494A.F494A.Scer\UAS} under the control of Scer\GAL4^elav.PLu results in an effective rescue of the ellipsoid body defects of Ptpmeg¹ mutants, while the ability of the transgene to rescue mushroom body defects is reduced compared to expression of a wild-type transgene.

(Whited et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9756

w^*; Ptpmeg¹/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ptpmeg¹

dptpmeg¹

(Li et al., 2015)

ptpmeg¹

(Hong et al., 2008, Whited et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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