FlyBase Allele Report: Dmel\Sema1a[Δcyto.UAS.Tag:MYC]

General Information

Symbol

Dmel\Sema1a^{Δcyto.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0194691

Feature type

allele

Associated gene

Dmel\Sema1a

Associated Insertion(s)

Carried in Construct

P{UAS-Sema1a.Δcyto}

Key Links

Transgenic product class

inframe_deletion

(Komiyama et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Komiyama et al., 2007)

Mutagen

in vitro construct

(Komiyama et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Sema1a.Δcyto}

Cytology

Description

UAS sequences drive expression of a truncated form of Sema1a that lacks the cytoplasmic domain but retains the extracellular and transmembrane domains. The construct has a Tag:MYC tag after the transmembrane domain-coding sequences.

(Komiyama et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Sema1a

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon & eye photoreceptor cell

(Cafferty et al., 2006)

axon & lamina

(Cafferty et al., 2006)

photoreceptor cell R1 & lamina

(Cafferty et al., 2006)

photoreceptor cell R2 & lamina

(Cafferty et al., 2006)

photoreceptor cell R3 & lamina

(Cafferty et al., 2006)

photoreceptor cell R4 & lamina

(Cafferty et al., 2006)

photoreceptor cell R5 & lamina

(Cafferty et al., 2006)

photoreceptor cell R7 & lamina

(Cafferty et al., 2006)

Detailed Description

Statement

Reference

Axonal projection of eye photoreceptor cells in the optic lobes is normal in late third instar Sema-1a^{Δcyto.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^elav-C155 larva, but when the driver Scer\GAL4^GMR.PF is used instead, the organisation of R1-6 growth cones in the lamina plexus is disrupted in a manner very similar to that seen in Sema-1a^k13702 homozygotes.

(Cafferty et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Sema1a^{Δcyto.UAS.Tag:MYC}/Scer\GAL4^GH146 fails to rescue Sema1a^k13702

(Komiyama et al., 2007)

Sema1a^{Δcyto.UAS.Tag:MYC}/Scer\GAL4^elav-C155 fails to rescue Sema1a^k13702/Df(2L)N22-5

(Cafferty et al., 2006)

Comments

The photoreceptor axon projection phenotype seen in the optic lobes of late third instar Sema-1a^k13702/Df(2L)N22-5 larvae is no rescued by Sema-1a^Scer\UAS.cYa; Scer\GAL4^elav-C155.

(Cafferty et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Sema-1a^{Δcyto.Scer\UAS.T:Hsap\MYC}

Sema1a^{Δcyto.Scer\UAS.T:Hsap\MYC}

Sema1a^{Δcyto.UAS.Tag:MYC}

sema-1a^Δcyto

(Komiyama et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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