UASt regulatory sequences drive expression of serp 5'UTR and coding sequences in which the polysaccharide deacetylase domain (Deac) is removed (amino acids 196-331), fused to a EGFP tag.
Tracheal expression of serpΔDeac.Scer\UAS.T:Avic\GFP-EGFP, under the control of Scer\GAL4btl.PS does not cause any detectable tube-extension phenotype but does affect protein secretion, with accumulation inside the tracheal cells.