FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\spiritRNAi.UAS.cKa
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General Information
Symbol
Dmel\spiritRNAi.UAS.cKa
Species
D. melanogaster
Name
FlyBase ID
FBal0196030
Feature type
allele
Associated gene
Dmel\spirit
Associated Insertion(s)
Carried in Construct
P{UAS-spirit.RNAi.K}
Key Links
Transgenic product class
RNAi_reagent
(Kambris et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Kambris et al., 2006)
Mutagen
in vitro construct
(Kambris et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-spirit.RNAi.K}
Cytology
Description

UASt regulatory sequences drive expression of a 500bp cDNA fragment from the 5' end of the spirit coding sequence, inserted as an inverted repeat (IR) in a modified pUAST vector, pUAST-R57, which includes a 282bp genomic fragment of Ret, containing introns 5 and 6 between the two IR fragments, to enhance the effect of RNAi.

(Kambris et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
spirit
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of spiritdsRNA.IR.Scer\UAS, under the control of Scer\GAL4da.G32 does not affect activation of melanization during pupariation, nor does it suppress lethality induced by the inactivation of Spn27A.

      (Kambris et al., 2006)

      Flies expressing spiritdsRNA.IR.Scer\UAS under the control of Scer\GAL4da.G32 exhibit an immune-deficiency phenotype similar to Tl pathway mutants: these flies are highly susceptible to Enterococcus faecalis (gram-positive) and fail to properly induce Drs after infection with Micrococcus luteus (gram-positive).

      Flies expressing spiritdsRNA.IR.Scer\UAS, under the control of Scer\GAL4da.G32 exhibit impaired immune response . They exhibit a dramatic loss of survival rate after infection with E.faecalis (gram-positive bacterium). This is mirrored to a lesser extent, when these flies are infected with B.bassiana (filamentous fungus) or C.albicans (yeast).

      Flies expressing spiritdsRNA.IR.Scer\UAS under the control of Scer\GAL4da.G32 exhibit an increased susceptibility to fungal infections.

      (Kambris et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      spiritRNAi.IR.UAS
      spiritRNAi.UAS.cKa
      spiritdsRNA.IR.Scer\UAS
      spiritdsRNA.IR.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)