FlyBase Allele Report: Dmel\grass[RNAi.UAS.cKa]

General Information

Symbol

Dmel\grass^RNAi.UAS.cKa

Species

D. melanogaster

Name

FlyBase ID

FBal0196031

Feature type

allele

Associated gene

Dmel\grass

Associated Insertion(s)

Carried in Construct

P{UAS-grass.RNAi.K}

Key Links

Transgenic product class

RNAi_reagent

(Kambris et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Kambris et al., 2006)

Mutagen

in vitro construct

(Kambris et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-grass.RNAi.K}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp cDNA fragment from the 5' end of the grass coding sequence, inserted as an inverted repeat (IR) in a modified pUAST vector, pUAST-R57, which includes a 282bp genomic fragment of Ret, containing introns 5 and 6 between the two IR fragments, to enhance the effect of RNAi.

(Kambris et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

grass

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^da.G32

(Kambris et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing grass^{dsRNA.IR.Scer\UAS} under the control of Scer\GAL4^Act5C.PI show comparable levels of mortality to wild type in response to mild wounding in 7-8 day old adults.

(Nam et al., 2012)

Expression of grass^{dsRNA.IR.Scer\UAS}, under the control of Scer\GAL4^da.G32 does not affect activation of melanization during pupariation, nor does it suppress lethality induced by the inactivation of Spn27A.

(Kambris et al., 2006)

Flies expressing grass^{dsRNA.IR.Scer\UAS} under the control of Scer\GAL4^da.G32 exhibit an immune-deficiency phenotype similar to Tl pathway mutants: these flies are highly susceptible to Enterococcus faecalis (gram-positive) and fail to properly induce Drs after infection with Micrococcus luteus (gram-positive).

Silencing of grass, through expression of grass^{dsRNA.IR.Scer\UAS}, under the control of Scer\GAL4^da.G32, does not impair Tl activation by fungus or yeast.

(Kambris et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

grass^RNAi.IR.UAS

grass^RNAi.UAS.cKa

grass^{dsRNA.IR.Scer\UAS}

grass^dsRNA.IR.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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