FlyBase Allele Report: Dmel\GckIII[RNAi.WIZ.UAS]

General Information

Symbol

Dmel\GckIII^RNAi.WIZ.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0197180

Feature type

allele

Associated gene

Dmel\GckIII

Associated Insertion(s)

Carried in Construct

P{UAS-GckIII.RNAi.WIZ}

Key Links

Transgenic product class

RNAi_reagent

(Friedman and Perrimon, 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Friedman and Perrimon, 2006)

Mutagen

in vitro construct

(Friedman and Perrimon, 2006)

Progenitor genotype

Carried in construct

P{UAS-GckIII.RNAi.WIZ}

Cytology

Description

UAS regulatory sequences drive expression of a GckIII hairpin.

(Friedman and Perrimon, 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

GckIII

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^ap-md544

(Friedman and Perrimon, 2006)

visible, with Df(3R)Exel6176, Scer\GAL4^Bx-MS1096

(Friedman and Perrimon, 2006)

visible, with Scer\GAL4^ap-md544

(Friedman and Perrimon, 2006)

visible, with Scer\GAL4^Bx-MS1096

(Friedman and Perrimon, 2006)

Phenotype Manifest In

wing, with Df(3R)Exel6176, Scer\GAL4^Bx-MS1096

(Friedman and Perrimon, 2006)

wing, with Scer\GAL4^ap-md544

(Friedman and Perrimon, 2006)

wing, with Scer\GAL4^Bx-MS1096

(Friedman and Perrimon, 2006)

wing disc, with Scer\GAL4^ap-md544

(Friedman and Perrimon, 2006)

Detailed Description

Statement

Reference

Expression of GckIII^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^ap-md544 or Scer\GAL4^Bx-MS1096 results in loss of wing tissue.

The loss of wing tissue phenotype when GckIII^{dsRNA.WIZ.Scer\UAS} is expressed under the control of Scer\GAL4^Bx-MS1096 is more severe if the flies carry two copies of GckIII^{dsRNA.WIZ.Scer\UAS} or in the presence of Df(3R)Exel6176/+.

Expression of GckIII^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^ap-md544 results in increased cell death in the wing disc.

(Friedman and Perrimon, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

GckIII^RNAi.WIZ.UAS is partially rescued by Scer\GAL4^ap-md544/GckIII^UAS.Tag:HA

(Friedman and Perrimon, 2006)

Not rescued by

GckIII^RNAi.WIZ.UAS is not rescued by Scer\GAL4^ap-md544/GckIII^{K24R.UAS.Tag:HA}

(Friedman and Perrimon, 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Used to study the phenotypic consequences of dsRNA interference (RNAi) of the GckIII gene.

(Friedman and Perrimon, 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

GckIII^RNAi.WIZ.UAS

GckIII^{dsRNA.WIZ.Scer\UAS}

GckIII^{dsRNA.WIZ.UAS}

dGCKIII^pWiz

(Friedman and Perrimon, 2006)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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