Expression of cbs^{GRIP.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} at 25^oC results in centrosome hypertrophy and asymmetric centrosome pairs in 37% of embryos during prophase. In an additional 15% of embryos, centrosome hypertrophy is associated with re-replication of individual centrosomes. Whenever centrosome hypertrophy is severe, there are free cytoplasmic centrosomes and an increase in the number of nuclei that are acentrosomal or have a single centrosome. Precocious replication of centrosomes is not seen during metaphase or anaphase.

If centrosome hypertrophy is severe, embryos expressing cbs^{GRIP.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} fail because of mitotic defects (including multipolar spindles and severe aneuploidy) prior to cellularization, although some viable animals are produced, even at 29^oC.