FlyBase Allele Report: Dmel\β4GalT7[RNAi.UAS.cGa]

General Information

Symbol

Dmel\β4GalT7^RNAi.UAS.cGa

Species

D. melanogaster

Name

FlyBase ID

FBal0197906

Feature type

allele

Associated gene

Dmel\β4GalT7

Associated Insertion(s)

Carried in Construct

P{UAS-β4GalT7.RNAi.G}

Key Links

Transgenic product class

RNAi_reagent

(Goda et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Goda et al., 2006)

Mutagen

in vitro construct

(Goda et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-β4GalT7.RNAi.G}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat of nucleotides 14-495 of the β4GalT7 coding sequence.

(Goda et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

β4GalT7

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^en-e16E

(Goda et al., 2006)

visible, with Scer\GAL4^GMR.PS

(Ueyama et al., 2008)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PS

(Ueyama et al., 2008)

interommatidial bristle, with Scer\GAL4^GMR.PS

(Ueyama et al., 2008)

ommatidium, with Scer\GAL4^GMR.PS

(Ueyama et al., 2008)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Goda et al., 2006)

Detailed Description

Statement

Reference

Flies expressing β4GalT7^{dsRNA.cGa.Scer\UAS} under the control of Scer\GAL4^GMR.PS have a weak rough eye phenotype, with disrupted ommatidial assembly, fused ommatidia and duplication and/or loss of interommatidial bristles.

(Ueyama et al., 2008)

100% of flies expressing β4GalT7^{dsRNA.cGa.Scer\UAS} under the control of Scer\GAL4^en-e16E show a reduction in size of the posterior compartment of the wing.

(Goda et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

β4GalT7^RNAi.UAS.cGa, Scer\GAL4^GMR.PS has visible phenotype, enhanceable by β3GalTII^RNAi.UAS, Scer\GAL4^GMR.PS

(Ueyama et al., 2008)

Enhancer of

Statement

Reference

β4GalT7^RNAi.UAS.cGa, Scer\GAL4^GMR.PS is an enhancer of visible phenotype of β3GalTII^RNAi.UAS, Scer\GAL4^GMR.PS

(Ueyama et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

β4GalT7^RNAi.UAS.cGa, Scer\GAL4^GMR.PS has interommatidial bristle phenotype, enhanceable by β3GalTII^RNAi.UAS, Scer\GAL4^GMR.PS

(Ueyama et al., 2008)

Enhancer of

Statement

Reference

β4GalT7^RNAi.UAS.cGa, Scer\GAL4^GMR.PS is an enhancer of interommatidial bristle phenotype of β3GalTII^RNAi.UAS, Scer\GAL4^GMR.PS

(Ueyama et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of both β3GalTII^{dsRNA.Scer\UAS} and β4GalT7^{dsRNA.cGa.Scer\UAS} under the control of Scer\GAL4^GMR.PS results in an enhanced rough eye phenotype compared to either single construct expressed alone; the double mutant flies show an increased penetrance of the abnormal interommatidial bristle phenotype.

(Ueyama et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Used to study the phenotypic consequences of dsRNA interference (RNAi) of the β4GalT7 gene.

(Goda et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

β4GalT7^RNAi.UAS.cGa

β4GalT7^RNAi.cGa.UAS

β4GalT7^{dsRNA.cGa.Scer\UAS}

β4GalT7^{dsRNA.cGa.UAS}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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