A 1.75kb dnc cDNA encoding the carboxy terminal part of the coding region (from the last 19 nucleotides of exon 5, through to the stop codon) is expressed under the control of Hsp70 promoter sequences (1140bp 5' flanking sequences and 250bp leader sequences).

Pde4^hs.PD rescues Pde4^dnc-1

Pde4^hs.PD partially rescues Pde4^dnc-1

The low learning score phenotype of dnc^ML is partially rescued by dnc^hs.PD expressed using heat shock, and fertility is restored to approximately 50% in these flies if dnc^hs.PD is expressed using heat shock. The low learning score phenotype of dnc¹ is partially rescued by dnc^hs.PD expressed using heat shock.

Embryos injected with the P{hsp-dnc} transformation construct die at or before the cellular blastoderm stage. Transformants have been obtained by coinjecting the P{hsp-dnc} transformation construct with a phosphodiesterase inhibitor.