FlyBase Allele Report: Dmel\αTub84B[GD17779]

General Information

Symbol

Dmel\αTub84B^GD17779

Species

D. melanogaster

Name

FlyBase ID

FBal0198551

Feature type

allele

Associated gene

Dmel\αTub84B

Associated Insertion(s)

Carried in Construct

P{GD17779}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD17779}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD17779

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

increased cell number | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Phenotype Manifest In

type II neuroblast | increased number | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

type I neuroblast | increased number | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Detailed Description

Statement

Reference

Expression of αTub84B^GD17779 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^insc-Mz1407 results in formation of ectopic neuroblast in both type I lineages and type II lineages in third instar larval brains.

Expression of αTub84B^GD17779 under the control of Scer\GAL4^erm.PU does not have any obvious effect on neuroblast numbers in third instar larval brains compared to controls.

(Zhang et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

αTub84B^GD17779, Scer\GAL4^insc-Mz1407 has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

αTub84B^GD17779, Scer\GAL4^insc-Mz1407 has increased cell number | third instar larval stage phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/αTub84B^GD17779 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of pins^P89

(Zhang et al., 2016)

Scer\GAL4^insc-Mz1407/αTub84B^GD17779 is a suppressor of decreased cell number | third instar larval stage phenotype of pins^P89

(Zhang et al., 2016)

Other

Statement

Reference

αTub84B^GD17779, Scer\GAL4^erm.PU, pins^P89 has abnormal neuroanatomy | third instar larval stage phenotype

(Zhang et al., 2016)

αTub84B^GD17779, Scer\GAL4^erm.PU, pins^P89 has increased cell number | third instar larval stage phenotype

(Zhang et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

αTub84B^GD17779, Scer\GAL4^insc-Mz1407 has type I neuroblast | third instar larval stage | increased number phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

αTub84B^GD17779, Scer\GAL4^insc-Mz1407 has type II neuroblast | third instar larval stage | increased number phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

Enhancer of

Statement

Reference

αTub84B^GD17779, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

NOT Enhancer of

Statement

Reference

αTub84B^GD17779, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

NOT Suppressor of

Statement

Reference

αTub84B^GD17779, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

Other

Statement

Reference

αTub84B^GD17779, Scer\GAL4^erm.PU, pins^P89 has intermediate neuronal progenitor | third instar larval stage phenotype

(Zhang et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of brain neuroblasts observed in third instar larvae expressing αTub84B^GD17779 under the control of Scer\GAL4^insc-Mz1407 is increased further by combination with pins^P89 (although the pins^P89 mutants on their own display significantly lower number of brain neuroblasts compared to controls) and leads to a dramatic neuroblast overgrowth.

Expression of αTub84B^GD17779 under the control of Scer\GAL4^erm.PU in pins^P89 mutant background leads to severely increased number of neuroblast due to dedifferentiation of intermediate neural progenitors.

(Zhang et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v52346

w¹¹¹⁸ P{GD17779}v52346

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

αTub84B^GD17779

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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