FlyBase Allele Report: Dmel\Gld2[GD6221]

General Information

Symbol

Dmel\Gld2^GD6221

Species

D. melanogaster

Name

FlyBase ID

FBal0204795

Feature type

allele

Associated gene

Dmel\Gld2

Associated Insertion(s)

Carried in Construct

P{GD6221}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6221}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6221

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | spermatogenesis, with Scer\GAL4^Tub.PU

(Sartain et al., 2011)

decreased cell number | spermatogenesis, with Scer\GAL4^VP16.bam

(Sartain et al., 2011)

female fertile, with Scer\GAL4^Tub.PU

(Sartain et al., 2011)

female fertile, with Scer\GAL4^VP16.bam

(Sartain et al., 2011)

male sterile, with Scer\GAL4^Tub.PU

(Sartain et al., 2011)

male sterile, with Scer\GAL4^VP16.bam

(Sartain et al., 2011)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

chromatin | pupal stage, with Scer\GAL4^Tub.PU

(Sartain et al., 2011)

chromatin | pupal stage, with Scer\GAL4^VP16.bam

(Sartain et al., 2011)

chromatin | spermatogenesis, with Scer\GAL4^Tub.PU

(Sartain et al., 2011)

chromatin | spermatogenesis, with Scer\GAL4^VP16.bam

(Sartain et al., 2011)

elongation stage spermatid | pupal stage, with Scer\GAL4^Tub.PU

(Sartain et al., 2011)

elongation stage spermatid | pupal stage, with Scer\GAL4^VP16.bam

(Sartain et al., 2011)

individualization stage spermatid, with Scer\GAL4^Tub.PU

(Sartain et al., 2011)

individualization stage spermatid, with Scer\GAL4^VP16.bam

(Sartain et al., 2011)

microtubule organizing center | spermatogenesis, with Scer\GAL4^Tub.PU

(Sartain et al., 2011)

microtubule organizing center | spermatogenesis, with Scer\GAL4^VP16.bam

(Sartain et al., 2011)

spermatid cyst, with Scer\GAL4^Tub.PU

(Sartain et al., 2011)

spermatid cyst, with Scer\GAL4^VP16.bam

(Sartain et al., 2011)

spermatozoon, with Scer\GAL4^Tub.PU

(Sartain et al., 2011)

spermatozoon, with Scer\GAL4^VP16.bam

(Sartain et al., 2011)

sperm individualization complex, with Scer\GAL4^Tub.PU

(Sartain et al., 2011)

sperm individualization complex, with Scer\GAL4^VP16.bam

(Sartain et al., 2011)

Detailed Description

Statement

Reference

Expression of Gld2^GD6221 under the control of Scer\GAL4^VP16.bam or Scer\GAL4^Tub.PU results in testes and seminal vesicles being devoid of mature sperm; fully elongated spermatogenic cysts contain syncytial spermatids, but do not contain bundled nuclei, assembled individualization complexes or basal bodies; elongated spermatids have bulges of material that accumulate along their tails. Expression of Gld2^GD6221 under the control of Scer\GAL4^VP16.bam or Scer\GAL4^Tub.PU results in less condensed nuclei with a more scattered distribution within the spermatocyst even at the earliest stages of spermatocyst elongation in the pupa, without any alteration in the number of nuclei, compared to controls.

(Sartain et al., 2011)

Adults expressing Gld2^GD6221 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v51605

w¹¹¹⁸; P{GD6221}v51605

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG5732^GD6221

Gld2^GD6221

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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