FlyBase Allele Report: Dmel\eEF5[GD8700]

General Information

Symbol

Dmel\eEF5^GD8700

Species

D. melanogaster

Name

FlyBase ID

FBal0209466

Feature type

allele

Associated gene

Dmel\eEF5

Associated Insertion(s)

Carried in Construct

P{GD8700}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8700}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8700

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

macrochaeta, with Scer\GAL4^Eq

(Patel et al., 2009)

wing cell, with Scer\GAL4^dpp.blk1

(Patel et al., 2009)

Detailed Description

Statement

Reference

Adults expressing eIF-5A^GD8700 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in undefined lethality.

(Schnorrer et al., 2010)

Knock-down of eIF-5A in the dpp expression stripes in the wing disc, through expression of eIF-5A^GD8700 under the control of Scer\GAL4^dpp.blk1 causes a narrowing of the region between the L3 and L4 wing veins.

Knock-down of eIF-5A^GD8700 in the thorax under the control of Scer\GAL4^Eq1 results in decreased bristle size on the thorax.

RNAi knock-down through expression of eIF-5A^GD8700 under the control of Scer\GAL4^tub severely affects larval growth.

Expression of eIF-5A^GD8700 in larval fat bodies under the control of Scer\GAL4^Adh.PF induces autophagy.

(Patel et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

eEF5^GD8700

eIF-5A^GD8700

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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