FlyBase Allele Report: Dmel\kra[1]

General Information

Symbol

Dmel\kra¹

Species

D. melanogaster

Name

FlyBase ID

FBal0212935

Feature type

allele

Associated gene

Dmel\kra

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Lee et al., 2007)

Progenitor genotype

P{lacW}kra^j9B6

(Lee et al., 2007)

Cytology

Description

Imprecise excision of the P{lacW} element, resulting in a 2068bp deletion of exba, corresponding to -1729 to +338 of the exba sequence.

(Lee et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with kra²)

(Sanchez-Soriano et al., 2009)

abnormal neuroanatomy | embryonic stage (with kra²)

(Lee et al., 2007)

female sterile | germline clone

(Lee et al., 2007)

Phenotype Manifest In

axon & pCC neuron (with kra²)

(Lee et al., 2007)

filopodium (with kra²)

(Sanchez-Soriano et al., 2009)

neuron (with kra²)

(Sanchez-Soriano et al., 2009)

Detailed Description

Statement

Reference

exba¹/exba² primary neuronal cultures show normal microtubule organization but a significant reduction in filopodia number.

(Sanchez-Soriano et al., 2009)

In exba¹/exba² embryos, the pCC axon aberrantly crosses the midline in 18% of CNS segments.

Females that contain homozygous exba¹ germline clones do not lay eggs as oogenesis is blocked.

(Lee et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

kra²/kra¹ has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by shot³/shot[+]

(Lee et al., 2007)

Enhancer of

Statement

Reference

kra²/kra¹ is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of shot³

(Lee et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

kra²/kra¹ has axon & pCC neuron phenotype, enhanceable by shot³/shot[+]

(Lee et al., 2007)

Enhancer of

Statement

Reference

kra²/kra¹ is an enhancer of axon & pCC neuron phenotype of shot³

(Lee et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of aberrant pCC axon midline crossovers in shot³/+; exba¹/exba² and shot³/shot³; exba²/+ double mutant embryos is significantly greater than in either single mutant homozygous embryos. The midline crossing phenotype is slightly more severe in shot³/shot³; exba¹/exba² embryos compared to shot³/shot³; exba²/+ embryos, while shot³/+; exba²/+ embryos show no significant midline defects.