FB2026_02 , released June 18, 2026
Allele: Dmel\wunUAS.EGFP
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General Information
Symbol
Dmel\wunUAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0213016
Feature type
allele
Associated gene
Dmel\wun
Associated Insertion(s)
Carried in Construct
P{UAS-wun.EGFP}
Key Links
Transgenic product class
wild_type
(Burnett and Howard, 2003)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Burnett and Howard, 2003)
Mutagen
in vitro construct
(Burnett and Howard, 2003)
Progenitor genotype
Carried in construct
P{UAS-wun.EGFP}
Cytology
Description

UASt regulatory sequences drive expression of wun that is tagged at the C-terminal end with EGFP.

(Burnett and Howard, 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
wun
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos expressing wunScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 have reduced numbers of germ cells due to germ cell death.

      (Ile et al., 2012)

      Embryos expressing wunScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4twi.PG show a reduction in the number of primordial germ cells (PGCs) and defects in PGC migration.

      (Burnett and Howard, 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of wunScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4btl.PS completely rescues the tracheal defects that are seen in embryos lacking both maternal and zygotic wun and wun2 function.

      Very few animals which lack both maternal and zygotic wun and wun2 function (derived from females containing double mutant wun49 wun2EP2650ex34 germline clones mated to Df(2R)wun-GL/+ or Ab(2;?)wunCE/+ males) survive to adulthood. This lethality is only partially rescued by expression of wunScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4btl.PS.

      (Ile et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in plasmid "Wun-GFP", transfected into S2 cells and used to study the catalytic activity of the protein produced.

      (Burnett and Howard, 2003)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      wunScer\UAS.T:Avic\GFP-EGFP
      wunUAS.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (4)