Rearing CaMKII^{T287D.Tn10\tetO}/+;Scer\GAL4^30Y/Tn10\tetR^{Scer\UAS.T:Hsim\VP16} flies either without tetracycline or with low doses (1microgram/ml), produces a suppression in male courtship initiation as compared to controls. With continual exposure to mated females, these flies suppress courtship immediately, unlike controls which show a lag. With the addition of Scer\GAL80^Cha.PK to these flies, males only show reduction in initial courtship of mated females compared to the controls.

Animals expressing CaMKII^{T287D.Tn10\tetO} under the control of Scer\GAL80^Cha.PK and Tn10\tetR^{Scer\UAS.T:Hsim\VP16} without tetracycline, show an enhanced training-dependent suppression of courtship with continued exposure to mated females, when compared to controls.

Expression of CaMKII^{T287D.Tn10\tetO} in combination with Tn10\tetR^{Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^29BD results in reduced viability and underinflated wings and abdomens. Phenotypes caused by CaMKII^{T287D.Tn10\tetO} expression can be rescued by suppression of the transgene via tetracycline feeding.

Males that express CaMKII^{T287D.Tn10\tetO} in combination with Tn10\tetR^{Scer\UAS.T:Hsim\VP16}, under the control of either Scer\GAL4^MJ85b or Scer\GAL4^30Y, show improved courtship training following 1 hour with mated females. These males start at a lower courtship level compared to wild-type males and are able to immediately begin to further suppress courtship. This phenotype is blocked by feeding with tetracycline and is never seen with the drivers Scer\GAL4^Tab2-201Y, Scer\GAL4^29BD or Scer\GAL4^MJ94. The short-term memory of CaMKII^{T287D.Tn10\tetO}, Tn10\tetR^{Scer\UAS.T:Hsim\VP16}, Scer\GAL4^MJ85b and CaMKII^{T287D.Tn10\tetO}, Tn10\tetR^{Scer\UAS.T:Hsim\VP16}, Scer\GAL4^30Y flies is not significantly different from controls and neither is their ability to suppress courtship during training with immature males.