FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ncdnk11.GFP(S65T)
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General Information
Symbol
Dmel\ncdnk11.GFP(S65T)
Species
D. melanogaster
Name
FlyBase ID
FBal0216901
Feature type
allele
Associated gene
Dmel\ncd
Associated Insertion(s)
Carried in Construct
P{ncdnk11-gfp*}
Key Links
Transgenic product class
missense_variant
(Sciambi et al., 2005)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Sciambi et al., 2005)
Mutagen
in vitro construct
(Sciambi et al., 2005)
Progenitor genotype
Carried in construct
P{ncdnk11-gfp*}
Cytology
Description

Amino acid replacement: N340K.

(Sciambi et al., 2005)

The ncd promoter drives expression of a mutant form of ncd ("NK11"), which is tagged at the C-terminal end with GFP(S65T).

(Sciambi et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
ncd
Encoded product / tool
ncd
Tagged with
GFP(S65T)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Meiotic spindles of females homozygous for ncdnk11.T:Avic\GFP-S65T in a ncd1 background do not differ significantly in overall appearance from those seen in females homozygous for ncdT:Avic\GFP-S65T in a ncd1 background.

      Early cleavage spindles and division kinetics are normal in embryos homozygous for ncdnk11.T:Avic\GFP-S65T in a ncd1 background. The pole-to-pole length of mitotic spindles at metaphase is greater in these embryos than in embryos homozygous for ncdT:Avic\GFP-S65T in a ncd1 background and the difference in length increased at anaphase. The anaphase spindles of embryos homozygous for ncdnk11.T:Avic\GFP-S65T in a ncd1 background frequently appear bent.

      (Sciambi et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      ncdnk11.GFP(S65T) rescues ncd1

      (Sciambi et al., 2005)
      Comments

      Two copies of ncdnk11.T:Avic\GFP-S65T rescue the chromosome missegregation defects of ncd1 females.

      (Sciambi et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ncdnk11.GFP(S65T)
      ncdnk11.T:Avic\GFP-S65T
      Name Synonyms
      Secondary FlyBase IDs
        References (1)