FB2026_03 , released September 17, 2026
Allele: Dmel\Fad2RNAi.UAS
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General Information
Symbol
Dmel\Fad2RNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0216907
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Transgenic product class
Nature of the Allele
Transgenic product class
Progenitor genotype
Carried in construct
Cytology
Description

UASt regulatory sequences drive expression of a 480bp Fad2 fragment and a 416bp Fad2 fragment, arranged as an inverted repeat in head-to-head orientation. The Fad2 sequences can form a 422bp dsRNA stem with a 73bp loop.

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Females expressing Fad2dsRNA.Scer\UAS under the control of Scer\GAL41407 show a 27% increase in linear saturated hydrocarbons compared to controls. The amount of dienes and pheromones is decreased by 98%, while that of monoenes is doubled.

Wild-type males show altered courtship behaviour towards females expressing Fad2dsRNA.Scer\UAS under the control of Scer\GAL41407 compared towards wild-type females; there are 12% fewer copulation attempts and 15% less copulation. Courtship, the first copulation attempt and copulation latencies are increased by 65%, 115% and 55% respectively.

Male and female flies expressing Fad2dsRNA.Scer\UAS under the control of Scer\GAL4OK72, Scer\GAL4shn-NP5250 or Scer\GAL4Lsp2.PH show no difference in the total amount of hydrocarbons compared to controls.

Females expressing Fad2dsRNA.Scer\UAS under the control of Scer\GAL4OK72 show a large decrease in 7,11-HD (-83%) and 7,11-ND (-85%) and an increase in 7-monoenes compared to controls.

Females expressing Fad2dsRNA.Scer\UAS under the control of Scer\GAL4shn-NP5250 show a significant decrease in diene percentages in favour of monoenes compared to controls.

Expression of Fad2dsRNA.Scer\UAS under the control of Scer\GAL4Lsp2.PH has no significant effect on the hydrocarbon profile of female flies.

Expression of Fad2dsRNA.Scer\UAS under the control of Scer\GAL4OK72, Scer\GAL4shn-NP5250 or Scer\GAL4Lsp2.PH has no significant effect on the hydrocarbon profile of male flies.

Wild-type males mated to females expressing Fad2dsRNA.Scer\UAS under the control of Scer\GAL4OK72 show a decrease in the number of copulation attempts and the courtship index and an increase in copulation latency compared to when wild-type males are mated to control females.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Fad2RNAi.UAS
Fad2dsRNA.Scer\UAS
Fad2dsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (4)