FlyBase curator comment: this entry is used to capture phenotypic information when the particular allele (or allele combination) used by the author could not be determined but the context of the experiment suggests that the phenotype being described is some kind of loss of function.
During recovery from etherisation after >10 minutes immobilization, Shabunspecified mutant adults exhibit continuous high frequency leg shaking. Recovery takes up to 10 minutes but is not (as with Sh mutants) accompanied by a wing buzz and is incomplete: once standing, the legs extend laterally and the animals drag their body along the floor while moving.
Wild-type larval locomotion across and agar plate leaves a trail of similar mouthook imprints at regular intervals. In contrast Shabunspecified mutant larvae leave trails in which the size of the imprint (force of hooks on the plate) varies greatly between imprints. These larvae often display uncoordinated local twitching of muscles in different segments interposed with long periods of pause and rarely show rhythmic contraction waves. The corresponding nerve spike activity recording from segmental nerves is also highly irregular - very different from the regular rhythmic activity seen in wild-type.
Within tens of seconds of 10Hz stimulation to a single segmental nerve in the larva, Shab mutants display a sudden jump in the amplitude of intracellularly recorded excitatory junctional potentials (ejps) in the stimulated nerve. The amplitude of this response is much greater than in wild-type, in which ejps gradually increase in amplitude and reach a low plateau level. These giant ejps are greater than those seen in sh mutants and, unlike in sh mutants are characterized by a prolonged duration and a slow rising phase with one or more notches. However, this response is not seen at Ca2+ levels greater than 0.5mM or at any Ca2+ concentrations with low frequency stimutation (<=0.5Hz).
In response to twin-pulse stimulation with an interpulse interval (IPI) between 20 and 100ms at 0.2mM Ca2+, Shab mutants exhibit a greater facilitation of neurotransmitter release resulting from the second pulse release than wild-type. This effect is especially pronounced at shorter IPIs.
From en passant recordings of action potentials at segmental nerves in the larva after 10 Hz stimulation: Shab mutants display supernumerary spikes coupled with a step-wise increase in excitatory junctional potential amplitudes. Neither of these effects are seen in wild-type or Sh mutants.