Homozygous clones induced at the late blastoderm/early gastrulation stage and then examined in the third larval instar tracheal system result in bending in the dorsal trunk. The clonal cells are misshapen and much smaller than neighbouring wild-type cells.

Homozygous embryos show breaks in the dorsal trunk, and the lumen in the dorsal trunk remains discontinuous throughout development (this phenotype may be due to a second site mutation on the chromosome).