UASp regulatory sequences drive expression of the MS11 Sxl transcript (the first ORF of Sxl), tagged with two copies of the Tag:MYC epitope, one just after the starting codon and the other at the NdeI restriction enzyme site (1364).
This construct produces tagged Sxl MS11 RNA with an intact 3'UTR.
This construct produces tagged Sxl MS11 RNA with an intact 3'UTR.