FlyBase Allele Report: Dmel\Pi3K92E[t.RBD.Tag:MYC]

General Information

Symbol

Dmel\Pi3K92E^{t.RBD.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0218869

Feature type

allele

Associated gene

Dmel\Pi3K92E

Associated Insertion(s)

Carried in Construct

P{Myc-Dp110^RBD}

Key Links

Transgenic product class

missense_variant

(Orme et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Orme et al., 2006)

Mutagen

in vitro construct

(Orme et al., 2006)

Progenitor genotype

Carried in construct

P{Myc-Dp110^RBD}

Cytology

Description

The endogenous Pi3K92E promoter drives expression of a genomic fragment of Pi3K92E containing four mutations in the Ras-binding domain (RBD) of the Pi3K92E protein (amino acid replacements T231D, K250A, R253A and K257A), thereby abolishing its ability to bind the Ras85D protein. The mutant protein is tagged with Tag:MYC.

(Orme et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

Pi3K92E

Encoded product / tool

Pi3K92E

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Pi3K92E^{t.RBD.Tag:MYC} rescues Pi3K92E^A

(Orme et al., 2006)

Partially rescues

Pi3K92E^{t.RBD.Tag:MYC} partially rescues Pi3K92E^A

(Orme et al., 2006)

Comments

The P{Myc-Dp110^RBD} transgene rescues Pi3K92E^A lethality, as assayed in "Df(3R)Pi3K92E^A, P{HBS}/Df(3R)Pi3K92E^A" mutants. The rescued flies are fertile and produce viable offspring. P{Myc-Dp110^RBD} also rescues Pi3K92E^A lethality when Pi3K21B dosage is halved, as assayed in "Df(2L)Pi3K21B-A/+; Df(3R)Pi3K92E^A, P{HBS}, P{U2af38.gR10}/Df(3R)Pi3K92E^A" mutants.

Pi3K92E^A homozygous null flies (genotype Df(3R)Pi3K92E^A, P{HBS}) rescued with 2 copies of the P{Myc-Dp110^RBD} transgene have wings approximately 86% of the area of control flies (Pi3K92E^A homozygous null flies rescued with 2 copies of the P{Myc-Dp110} transgene), while their body mass is reduced to approximately 69% of controls.

Pi3K92E^A homozygous null flies (genotype Df(3R)Pi3K92E^A, P{HBS}) rescued with 2 copies of the P{Myc-Dp110^RBD} transgene lay fewer eggs than controls (Pi3K92E^A homozygous null flies rescued with 2 copies of the P{Myc-Dp110} transgene) at ages. Consistent with this, Pi3K92E^A homozygous null flies rescued with 2 copies of P{Myc-Dp110^RBD} have smaller ovaries with fewer ovarioles than controls.