Simultaneous expression of DJ-1α^{a.Sym.Scer\UAS} and DJ-1α^{b.Sym.Scer\UAS}, under the control of Scer\GAL4^da.G32 (to generate dsRNA) generates mutant flies that exhibit similar climbing ability to control flies. However, this ability declines faster in mutants than in control flies.

Simultaneous expression of DJ-1α^{a.Sym.Scer\UAS} and DJ-1α^{b.Sym.Scer\UAS}, under the control of Scer\GAL4^da.G32 (to generate dsRNA) generates mutant flies that display a significantly shorter lifespan than control flies. The mean lifespan for dj-1β^A286 flies is 17 days, while control flies exhibit a mean lifespan greater than 38. However, both mutant and control flies exhibit a similar maximum lifespan.

Simultaneous expression of DJ-1α^{a.Sym.Scer\UAS} and DJ-1α^{b.Sym.Scer\UAS}, under the control of Scer\GAL4^da.G32 (to generate dsRNA) generates mutant flies that are more sensitive to paraquat treatment than age-matched control flies.

Simultaneous expression of DJ-1α^{a.Sym.Scer\UAS} and DJ-1α^{b.Sym.Scer\UAS}, under the control of Scer\GAL4^da.G32 (to generate dsRNA) does not affect viability, fertility, or indeed generate any external phenotype.

Simultaneous expression of DJ-1α^{a.Sym.Scer\UAS} and DJ-1α^{b.Sym.Scer\UAS}, under the control of Scer\GAL4^ple.PF (to generate dsRNA) does not significantly affect the number of DA neurons in 1-day old and 30-day old flies. A little but significant difference is observed between control flies and flies with DA specific inhibition of DJ-1α at 30 days of age. The morphology of these neurons is also normal.

Expression of DJ-1α^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^da.G32 (to generate dsRNA) generates short lived flies. Although newly eclosed adults exhibit similar climbing ability to control flies, this ability declines faster than in control flies.