FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\sing23
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General Information
Symbol
Dmel\sing23
Species
D. melanogaster
Name
FlyBase ID
FBal0221652
Feature type
allele
Associated gene
Dmel\sing
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Estrada et al., 2007)
Mutagen
ethyl methanesulfonate
(Estrada et al., 2007)
Progenitor genotype
Cytology
Description

Amino acid replacement: W142term.

(Estrada et al., 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:16,679,882..16,679,882 [+]
Nucleotide change:

G16679882A

Amino acid change:

W142term | sing-PA

Reported amino acid change:

W142term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Estrada et al., 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Stage 14 homozygous embryos have unfused groups of myoblasts, reminiscent of a "bunch of grapes" phenotype (wild-type embryos have no unfused myoblasts at this stage).

      Stage 12 homozygous embryos contain the normal number of Kr-positive founder cells and lmd-positive fusion-competent myoblasts.

      DA1 muscles are uniformly binucleate in homozygous stage 16 embryos (wild-type DA1 muscles contain 9-14 nuclei at this stage).

      Ultrastructural analysis of adhering myoblasts that are in the process of fusing in homozygous embryos indicates that they contain pre-fusion complexes (electron-dense vesicles paired on either side of the fusing plasma membranes) as occurs in wild-type embryos. However, the number of pre-fusion complexes in the myoblasts of late stage 12/early stage 13 mutant embryos is significantly higher than in wild-type embryos; on average 48% of mutant myoblasts contain pre-fusion complexes compared to 22% of wild-type myoblasts. The total number of vesicles pr pre-fusion complex is slightly but significantly higher in the mutant embryos compared to wild type.

      (Estrada et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      sing23
      (Bothe et al., 2014, Estrada et al., 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)