FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Vps28D2
Open Close
General Information
Symbol
Dmel\Vps28D2
Species
D. melanogaster
Name
FlyBase ID
FBal0230152
Feature type
allele
Associated gene
Dmel\Vps28
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Vaccari et al., 2009, Rusten et al., 2007)
Progenitor genotype
Cytology
Description

Amino acid replacement: R102term.

(Vaccari et al., 2009)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:8,079,647..8,079,647 [-]
Nucleotide change:

C8079647T

Reported nucleotide change:

C?T

Amino acid change:

R102term | Vps28-PA; R102term | Vps28-PB

Reported amino acid change:

R102term

(Vaccari et al., 2009)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  Huntington's disease
      modeled by Hsap\HTTGMR.Q120
      (Rusten et al., 2007)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Somatic clones of Vps28D2/Vps28D2 cells in the third instar larval eye disc display low levels of apoptosis (detected by Caspase-3 staining) and show abnormal localization of rho in endosomes.

      (Sheng et al., 2016)

      Homozygous Vps28D2 mutant tissue partially survives in mosaic eye discs, and is not completely 'out-competed'. Although mutant ommatidia are not recovered, the wild-type ommatidia composing the adult eyes of mosaic animals are variably overgrown.

      Vps28D2 mutants exhibit neoplastic tumours.

      Compared with wild-type, egg chambers containing Vps28B9 mutant cells exhibit a loss of plasma membrane, ultimately leading to the formation of clusters of multinucleated cells enclosed by a single actin cytoskeleton, with nuclei rested around clumps of actin-positive structures, possibly also membrane fragments.

      (Vaccari et al., 2009)

      Gut cells of mutant larvae show strong accumulation of autophagosomes containing cytoplasmic material, in contrast to control larvae which have few autophagic structures in the gut.

      (Rusten et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Vps28[+]/Vps28D2 is an enhancer of eye photoreceptor cell phenotype of Hsap\HTTGMR.Q120

      (Rusten et al., 2007)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The loss of photoreceptors that is seen in flies expressing Hsap\HDGMR.Q120 is enhanced if the flies also carry one copy of Vps28D2.

      (Rusten et al., 2007)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Vps28D2
      (Sheng et al., 2016)
      vps28D2
      (Mukherjee et al., 2016, Vaccari et al., 2009, Rusten et al., 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)