FlyBase Allele Report: Dmel\Dnaaf5[KK102625]

General Information

Symbol

Dmel\Dnaaf5^KK102625

Species

D. melanogaster

Name

FlyBase ID

FBal0231900

Feature type

allele

Associated gene

Dmel\Dnaaf5

Associated Insertion(s)

Carried in Construct

P{KK102625}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK102625}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK102625

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of primary ciliary dyskinesia

CEA

(Diggle et al., 2014)

model of primary ciliary dyskinesia 18

CEA

(Zur Lage et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal auditory perception | larval stage, with Scer\GAL4^sca-DB

(Diggle et al., 2014)

abnormal locomotor behavior | adult stage, with Scer\GAL4^sca.PU

(Zur Lage et al., 2019)

male sterile, with Scer\GAL4^VP16.bam

(Zur Lage et al., 2019, Diggle et al., 2014)

uncoordinated | adult stage, with Scer\GAL4^sca-DB

(Diggle et al., 2014)

viable, with Scer\GAL4^sca-DB

(Diggle et al., 2014)

Phenotype Manifest In

inner dynein arm, with Scer\GAL4^sca-DB

(Diggle et al., 2014)

inner dynein arm, with Scer\GAL4^VP16.bam

(Diggle et al., 2014)

Johnston organ neuron, with Scer\GAL4^sca-DB

(Diggle et al., 2014)

outer dynein arm, with Scer\GAL4^sca-DB

(Diggle et al., 2014)

outer dynein arm, with Scer\GAL4^VP16.bam

(Diggle et al., 2014)

spermatid, with Scer\GAL4^VP16.bam

(Diggle et al., 2014)

spermatozoon, with Scer\GAL4^VP16.bam

(Zur Lage et al., 2019, Diggle et al., 2014)

Detailed Description

Statement

Reference

Expressing HEATR2^KK102625 under the control of Scer\GAL4^VP16.bam results in immotile sperm.

Adults expressing HEATR2^KK102625 under the control of Scer\GAL4^sca.PU show defective climbing activity, as compared to controls.

(Zur Lage et al., 2019)

Whereas control larvae contract abruptly when exposed to a 10kHz tone, Scer\GAL4^sca-DB HEATR2^KK102625 (+ UAS-Dcr2) larvae fail to respond.

The specification and gross differentiation of Ch neurons in Scer\GAL4^sca-DB HEATR2^KK102625 (+ UAS-Dcr2) flies is unaffected and gross formation of Ch neuron cilia is not disrupted.

The normal 9+0 arrangement of microtubule doublets in the proximal (motile) zone of the Ch neuron cilium in Scer\GAL4^sca-DB HEATR2^KK102625 (+ UAS-Dcr2) adult flies is present but the axoneme lacks both outer and inner dynein arms. Structures consistent with remnant dynein arms are only ever detected on a small minority of doublets.

Scer\GAL4^{bam.T:Hsim\VP16} HEATR2^KK102625 (+ UAS-Dcr2) testes appear to develop normally and mature sperm are present, but no motile sperm are observed in the seminal vesicle. While wild type cysts contain 64 spermatids, Scer\GAL4^{bam.T:Hsim\VP16} HEATR2^KK102625 (+ UAS-Dcr2) cysts contain an average of 60.7 identifiable spermatids. These have a normal axonemal ‘‘9+2’’ arrangement, but dynein arms are generally not visible.

(Diggle et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v106702

P{KK102625}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG31320^KK102625

Dnaaf5^KK102625

HEATR2^KK102625

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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