The expression of jusJF03192 leads to an abnormal paralysis phenotype upon exposure to 10[o]C temperatures (full penetrance if driven by Scer\GAL4elav-C155 or Scer\GAL4ChAT.7.4, high penetrance if driven by Scer\GAL4VGAT.PF and low penetrance if driven by Scer\GAL4VGlut-OK371), which is associated with a significant increase in recovery time from paralysis upon shift to room temperature, as compared to wild-type controls. The Scer\GAL4elav-C155-driven expression also leads to a bang sensitive phenotype if raised at 29[o]C.
Flies expressing jusJF03192 under the control of any of the following: Scer\GAL4ChAT.7.4, Scer\GAL4elav-C155 and Scer\GAL4Act5C.PI are almost all bang sensitive, Scer\GAL4VGAT.PF-driven expression results in a less penetrant phenotype, while expression controlled by either Scer\GAL4VGlut-OK371 or Scer\GAL4repo does not lead to any mechanical shock-induced paralysis. The median time of recovery from the paralysis ranges from ~50 min (with the Scer\GAL4ChAT.7.4 driver) to ~200 min (with Scer\GAL4Act5C.PI).
Expression of jusJF03192 under the control of Scer\GAL4Act5C.PI (combined with tub-Gal80[ts]) kept at moderate levels by keeping the animals at 22[o]C and then increased by shifting the flies to 29[o]C at various points of development (second larval instar, third larval instar, early pupal stage, late pupal stage, eclosion) leads to bang sensitive phenotype in adult flies but the penetrance of the phenotype as well as their median recovery time is significantly lower in flies that have been transfered late in development (as late pupae or adults).