FlyBase Allele Report: Dmel\nero[1]

General Information

Symbol

Dmel\nero¹

Species

D. melanogaster

Name

FlyBase ID

FBal0240589

Feature type

allele

Associated gene

Dmel\nero

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Patel et al., 2009)

Progenitor genotype

nero^s1921

(Patel et al., 2009)

Cytology

Description

Imprecise excision of P{lacW}nero^s1921 generates a small deletion that removes 189bp of the nero open-reading frame.

(Patel et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle | somatic clone

(Patel et al., 2009)

abnormal cell size | somatic clone

(Patel et al., 2009)

lethal - all die before end of second instar larval stage

(Patel et al., 2009)

lethal - all die before end of second instar larval stage (with Df(3R)04661)

(Patel et al., 2009)

some die during second instar larval stage

(Patel et al., 2009)

some die during second instar larval stage (with Df(3R)04661)

(Patel et al., 2009)

Phenotype Manifest In

embryonic/larval fat body | somatic clone

(Patel et al., 2009)

microchaeta | somatic clone

(Patel et al., 2009)

photoreceptor neuron | somatic clone

(Patel et al., 2009)

rhabdomere | somatic clone

(Patel et al., 2009)

trichome | somatic clone

(Patel et al., 2009)

Detailed Description

Statement

Reference

nero¹ mutants die as second instar (L2) larvae, whether they are homozygous or in heteroallelic combinations ((with Df(3R)04661).

nero¹ mutants cause both mild bristle loss and a severe reduction in bristle size. nero¹ mutant socket cells are specified but fail to reach their mature size. Cells adjacent to nero¹ exhibit mutant bristles that are smaller in size as indicated by trichome size and spacing. Ectodermal cells that lack nero in the developing thoraxes 47APF are significantly smaller than adjacent wild-type cells.

Rhabdomere size in nero¹ mutant eye clones are also reduced when compared with adjacent wild-type cells, suggesting a defect in photoreceptor size.

nero¹ mutant clones generated using the Scer\FLP1^ey.PN technique are generally irrecoverable. However, nero¹ mutant clones are easily obtained using Scer\FLP1^ey.PN when cell competition is ameliorated through the use of an FRT chromosome bearing a cell lethal mutation that effectively removes the mitotic wild-type, sister twin cells. Indeed, nero¹ mutant clones generated using this technique completely overtake the eye, leading to eyes and heads that are much smaller than wild-type heads.

nero¹ mutant clones generated in the wing imaginal disc are smaller and contain fewer cells than their associated wild-type twin spots. The differences in cell number between nero¹ clones and their wild-type twin spots are less obvious in small clones and clearly increase as clones get larger. This is likely caused by differences in nero protein perdurance in small clones. nero mutant cells are most likely eliminated because of an inability to compete with wild-type cells.

nero¹ mutant larval fat bodies prominently display enlarged, acidified structures, even when the animals are fed an amino acid source, suggesting that nero¹ mutant larvae undergo a constitutive starvation response in the presence of an amino acid source.

nero¹ mutant clones show a decreased level of BrdU incorporation, indicating a delay in cell cycle progression.

(Patel et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

nero¹ is an enhancer of decreased cell size phenotype of eEF5⁰¹²⁹⁶

(Patel et al., 2009)

Suppressor of

Statement

Reference

nero¹ is a suppressor of abnormal developmental rate phenotype of Tsc1^Q87X

(Patel et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

nero¹ has microchaeta | somatic clone phenotype, suppressible by Hsap\DOHH^UAS.cPa/Scer\GAL4^Tub.PU

(Patel et al., 2009)

NOT suppressed by

Statement

Reference

nero¹ has microchaeta | somatic clone phenotype, non-suppressible by eEF5^UAS.cPa/Scer\GAL4^Tub.PU

(Patel et al., 2009)

nero¹ has microchaeta | somatic clone phenotype, non-suppressible by Scer\GAL4^Tub.PU/Hsap\DOHH^H56A.UAS

(Patel et al., 2009)

nero¹ has microchaeta | somatic clone phenotype, non-suppressible by Hsap\DOHH^H89A.UAS/Scer\GAL4^Tub.PU

(Patel et al., 2009)

nero¹ has microchaeta | somatic clone phenotype, non-suppressible by Scer\GAL4^Tub.PU/Hsap\DOHH^H208A.UAS

(Patel et al., 2009)

nero¹ has microchaeta | somatic clone phenotype, non-suppressible by Hsap\DOHH^H240A.UAS/Scer\GAL4^Tub.PU

(Patel et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

eIF-5A⁰¹²⁹⁶; nero¹ double mutant larvae display a slightly more severe larval growth defect than eIF-5A⁰¹²⁹⁶ mutants alone.

Unlike either nero¹ or Tsc1^Q87X mutant clones, Tsc1^Q87X nero¹ double mutant clones cannot be recovered. Tsc1^Q87Xfully suppresses the autophagic defect seen in nero¹ mutant animals. However, this epistatic relationship is reveresed in other contexts such as larval growth and developmental timing. Tsc1^Q87X mutant larvae appear to undergo precocious development. However, this precocious development is suppressed in Tsc1^Q87X nero¹ double mutant larvae.

Overexpression of eIF-5A^Scer\UAS.cPa (under the control of Scer\GAL4^tub) is not sufficient to rescue bristle size defects associated with nero¹ mutant clones.

(Patel et al., 2009)

Xenogenetic Interactions

Statement

Reference

Overexpression of Hsap\DOHH^Scer\UAS.cPa fully complements defects in bristle size associated with nero¹ mutant clones on the thorax and does not cause any other obvious phenotype.

Overexpression of Hsap\DOHH^{H56A.Scer\UAS} fails to suppress the defects in bristle size associated with nero¹ mutant clones on the thorax and does not cause any other obvious phenotype.

Overexpression of Hsap\DOHH^{H89A.Scer\UAS} fails to suppress the defects in bristle size associated with nero¹ mutant clones on the thorax and does not cause any other obvious phenotype.

Overexpression of Hsap\DOHH^{H208A.Scer\UAS} fails to suppress the defects in bristle size associated with nero¹ mutant clones on the thorax and does not cause any other obvious phenotype.

Overexpression of Hsap\DOHH^{H240A.Scer\UAS} fails to suppress the defects in bristle size associated with nero¹ mutant clones on the thorax and does not cause any other obvious phenotype.

(Patel et al., 2009)

Complementation and Rescue Data

Rescued by

nero¹ is rescued by nero^+t1.5

(Patel et al., 2009)

nero¹ is rescued by nero^UAS.cPa/Scer\GAL4^Act5C.PP

(Patel et al., 2009)

Comments

Overexpression of eIF-5A^Scer\UAS.cPa (under the control of Scer\GAL4^tub) is not sufficient to rescue bristle size defects associated with nero¹ mutant clones.

(Patel et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

39728

P{hsFLP}1, y¹ w^*; P{neoFRT}82B nero¹/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

nero¹

(Patel et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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