FlyBase Allele Report: Dmel\CTCF[UAS.EGFP]

General Information

Symbol

Dmel\CTCF^UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0241204

Feature type

allele

Associated gene

Dmel\CTCF

Associated Insertion(s)

Carried in Construct

P{UAS-CTCF-EGFP}

Key Links

Transgenic product class

wild_type

(Mohan et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Mohan et al., 2007)

Mutagen

in vitro construct

(Mohan et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-CTCF-EGFP}

Cytology

Description

UAS regulatory sequences drive expression of CTCF coding sequences fused with EGFP sequences at the C-terminal end.

(Mohan et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

CTCF

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage, with Scer\GAL4^Act5C.PI

(Mohan et al., 2007)

lethal - all die during embryonic stage, with Scer\GAL4^αTub84B.PL

(Mohan et al., 2007)

some die during embryonic stage, with Scer\GAL4^Act5C.PI

(Mohan et al., 2007)

some die during embryonic stage, with Scer\GAL4^αTub84B.PL

(Mohan et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

Ubiquitous expression of CTCF^{Scer\UAS.T:Avic\GFP-EGFP} in a wild-type background with the drivers Scer\GAL4^αTub84B.PL or Scer\GAL4^Act5C.PI results in early embryonic lethality.

(Mohan et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

A single heat shock (37[o]C) for 20 mins at 24, 48, or 72 hours into development is sufficient to phenotypically rescue the majority of flies with a CTCF^P30.6/Df(3L)0463 or CTCF^GE24185/Df(3L)0463 background when CTCF^{Scer\UAS.T:Avic\GFP-EGFP} and Scer\GAL4^hs.PB are present. Both sexes are fertile. A few flies exhibit a very mild held-out wing phenotype and some have a disorganized bristle pattern on the dorsal abdomen. Heat shock at the pupal stage could not rescue all the mutant phenotypes.

(Mohan et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CTCF^{Scer\UAS.T:Avic\GFP-EGFP}

CTCF^UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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