FlyBase Allele Report: Dmel\Dcr-1[LL06357]

General Information

Symbol

Dmel\Dcr-1^LL06357

Species

D. melanogaster

Name

FlyBase ID

FBal0241469

Feature type

allele

Associated gene

Dmel\Dcr-1

Associated Insertion(s)

PBac{SAstopDsRed}Dcr-1^LL06357

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

PBac{SAstopDsRed}Dcr-1^LL06357

Cytology

Description

Insertion in the first exon, 2220bp 3' of the start codon.

(Berdnik et al., 2008)

Allele components

Component

Use(s)

Inserted element

PBac{SAstopDsRed}

gene trap

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:22,738,807..22,738,807 [-]

(Berdnik et al., 2008, Berdnik et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone

(Berdnik et al., 2008, Berdnik et al., 2008)

Phenotype Manifest In

adult antennal lobe projection neuron DL1 adPN | somatic clone

(Berdnik et al., 2008)

antennal lobe projection neuron adPN | somatic clone

(Berdnik et al., 2008)

antennal lobe projection neuron of ALl1 lineage | somatic clone

(Berdnik et al., 2008)

axon & DL1 adPN | somatic clone

(Berdnik et al., 2008)

dendrite & ad PN | somatic clone

(Berdnik et al., 2008)

dendrite & DA1 lPN | somatic clone

(Berdnik et al., 2008)

dendrite & DL1 adPN | somatic clone

(Berdnik et al., 2008)

dendrite & lPN | somatic clone

(Berdnik et al., 2008)

dendrite & vPN | somatic clone

(Berdnik et al., 2008)

oocyte | germline clone

(Azzam et al., 2012)

Detailed Description

Statement

Reference

7 days old females carrying homozygous germline clones result in egg chambers with 8 nurse cells and no oocyte with 30% penetrance.

(Azzam et al., 2012)

Homozygous single cell DL1 neuron clones mistarget to other glomeruli besides their normal DL1 target.

Homozygous adPN and lPN clones show a significant reduction in the dendritic mass compared to wild type and dendrites spill non-specifically into inappropriate glomeruli.

(Berdnik et al., 2008)

Homozygous projection neuron (PN) neuroblast MARCM clones labelled using Scer\GAL4^GH146 show dendritic defects, with a reduction in dendrite density and dendrites that innervate incorrect glomeruli.

Homozygous single-cell "DL1" projection neuron clones show stereotyped mistargeting defects in 19/35 cases examined; the dendrites innervate the DL glomerulus, but also mistarget several additional glomeruli (VA7m, VC2, VA6, DL2d and DL5). 7/35 of the remaining DL1 single cell projection neuron clones spill their dendrites medially and the remaining 9/35 target normally.

Homozygous projection neuron MARCM clones labelled using Scer\GAL4^Mz19 show sparse innervation of the VA1d glomerulus and the dendrites are incorrectly targeted to variable glomeruli such as DA3, D and DA2. 7/9 clones target lPNs normally to DA1 with wild-type dendrite densities, while 2/9 show additional partial innervation of the adjacent DL3 glomerulus.

The axons of homozygous single-cell "DL1" projection neuron clones project into the lateral horn (as occurs in wild type), but the main branches do not reach the edge of the lateral horn. In addition, the dorsal branch of the lateral horn projection is shorter than normal or absent.

(Berdnik et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Dcr-1^LL06357 has abnormal neuroanatomy | somatic clone phenotype, non-suppressible by Dcr-2^UAS.cDa/Scer\GAL4^GH146

(Berdnik et al., 2008)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Dcr-1^LL06357 has antennal lobe projection neuron adPN | somatic clone phenotype, non-suppressible by Dcr-2^UAS.cDa/Scer\GAL4^GH146

(Berdnik et al., 2008)

Dcr-1^LL06357 has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype, non-suppressible by Dcr-2^UAS.cDa/Scer\GAL4^GH146

(Berdnik et al., 2008)

Dcr-1^LL06357 has antennal lobe projection neuron of ALl1 lineage | somatic clone phenotype, non-suppressible by Dcr-2^UAS.cDa/Scer\GAL4^GH146

(Berdnik et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The mutant dendritic phenotypes of Dcr-1^LL06357 DL1 single neuron, adPN and lPN clones are not rescued or altered by expression of Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^GH146.

(Berdnik et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Dcr-1^LL06357 is rescued by Dcr-1^UAS.cDa/Scer\GAL4^GH146

(Berdnik et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

141894

y^* w^*; P{FRT(w^hs)}2A P{neoFRT}82B PBac{SAstopDsRed}Dcr-1^LL06357 P{Car20y}96E / TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Precise excision of the insertion reverts the projection neuron morphogenesis defects seen in homozygous clones.

(Berdnik et al., 2008)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dcr-1^LL06357

Dicer-1^LL06357

(Berdnik et al., 2008, Berdnik et al., 2008)

dcr-1^LL06357

(Azzam et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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