FB2026_02 , released June 18, 2026
Allele: Dmel\CG10283K01
Open Close
General Information
Symbol
Dmel\CG10283K01
Species
D. melanogaster
Name
FlyBase ID
FBal0241774
Feature type
allele
Associated gene
Dmel\CG10283
Associated Insertion(s)
TI{TI}CG10283K01
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
FLPase
(Sokol et al., 2008)
recombination
(Sokol et al., 2008)
I-SceI
(Sokol et al., 2008)
ends-in gene targeting
(Sokol et al., 2008)
Progenitor genotype
PBac{WH}CG10283f03195
(Sokol et al., 2008)
Associated Insertion(s)
TI{TI}CG10283K01
Cytology
Description

The P{FRT(Target70,let7CKO1)} has been used as a donor construct for end-in homologous recombination of the genomic region containing mir-100, let-7 and mir-125, which should result in partial duplication of the region, and an FRT site from the donor construct becoming inserted at this region of the genome in the targeted chromosome. This has been carried out in flies carrying PBac{WH}CG10283f03195, so that if this partial duplication is created, recombination can occur between the FRT site in the targeted chromosome and the FRT site in the insertion, resulting in a deletion of the sequence between the two sites. The end result is a deletion of approximately 1.1kb that removes the sequences encoding the mature mir-100, let-7 and mir-125 microRNAs, plus the CG10283 gene is also disrupted as a result of the recombination events.

(Sokol et al., 2008)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      Associated with: Df(2L)let-7-CKO1.

      (Sokol et al., 2008)

      The "let-7-C[KO1]" chromosome carries a 1071bp deletion that removes the mir-100, let-7 and mir-125 microRNAs. The CG10283 gene is also disrupted on this chromosome.

      (Sokol et al., 2008)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      CG10283K01
      Name Synonyms
      Secondary FlyBase IDs
        References (2)