Consists of the ncd promoter and ncd cDNA fused to mRFP1. The last four amino acids of wild-type ncd are -NFDK. This was changed to -NFLK-mRFP1. The construct was made in pP{CaSpeR-3}.
Native ncd promoter regulates expression of full-length ncd, fused at the C-terminus to mRFP1.